汉坦病毒感染体外诱导VeroE6细胞热休克蛋白的表达  

The expression of heat shock proteins in the veroE6 cells induced by Hantaan virus infection in vitro

作　　者：叶苓[1] 廖文俊[1] 刘彦仿[1] 杨守京[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2000年第4期399-401,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目!(39770664)

摘　　要：目的　研究汉坦病毒 (HTV)感染后对细胞应激基因表达的影响 .方法　用 HTV感染其敏感细胞株 Vero E6细胞 ,用 Western印迹杂交、RNA斑点杂交及原位杂交分别分析热休克蛋白 (HSP)的表达及编码 HSP的 m RNA水平的变化 .结果　病毒感染后细胞内 HSP70及热休克蛋白 m RNA水平均有增高 ,而 HSP6 5及 HSP2 7的表达则无明显改变 .结论　 HTV感染可以直接诱导 HSP70的高表达 。AIM To investigate the effect of Hantaan virus infection on the expression of stress genes. METHODS Virus infection, Western blot, RNA dot blot and in situ hybridization were employed. RESULTS The expression of HSP70 was enhanced. The level of HSP70 mRNA was increased,but the expression of HSP65 and HSP27 remained unchanged. CONCLUSION The overexpression of HSP70 can be induced directly by Hantaan virus, which may play a role in the protection of cells from virus infection.

关 键 词：热休克蛋白 汉坦病毒 VeroE6细胞 HTV感染 

分 类 号：R512.8[医药卫生—内科学] R373.32[医药卫生—临床医学]