黄颡鱼RAPD标记及其遗传多样性的初步分析  

RAPD Markers and Genetic Diversity in Pelteobagrus Fulvidraco

作　　者：宋平[1] 潘云峰[1] 向筑[1] 胡珈瑞[2] 胡隐昌[3] 蔡从利[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]武汉大学中南医院,湖北武汉430071 [3]中国水产科学研究院珠江水产研究所,广东广州510380

出　　处：《武汉大学学报（自然科学版）》2001年第2期233-237,共5页Journal of Wuhan University(Natural Science Edition)

基　　金：国家自然科学基金! (39770 5 91) ;第三世界科学院 (TWAS;Italy)研究基金! (95 -348RG/BIO/AS) ;珠江水产研究所农业部重点开放实验

摘　　要：以雌、雄黄颡鱼 DNA池为模板 ,从 42 2个引物中筛选出 6 1个 RAPD反应重复性好、带型清晰明亮的引物 ,这些引物可用于黄颡鱼的分子标记或鉴定 .采用 10～ 11个引物先后对两批黄颡鱼 (简称群体 A和群体 B)进行群体 RAPD多样性分析 .在群体 A(2 1尾 )中共检测到 95个位点 ,其中 2 7个为多态位点占总位点数2 8.42 % ,其群体 Nei& L i氏遗传相似率为 90 .6 7% ,Shannon信息指数为 0 .0 918比特 ;在群体 B中共检出 46个位点 ,其中多态位点 12个占 2 6 .0 9% ,其群体 Nei& L i氏遗传相似率为 93.2 7% ,Shannon信息指数为 0 .0 6 70比特 .primers whose RAPD reactions are reproductive and with clean bands are selected from 422 primers by using the DNA pools of both male and female Pelteobagrus fulvidraco as templates. These 61 primers can be used as molecular marker for the species identification. We apply 10\|11 primers to proceed RAPD analysis consequently to two groups. In the first group, 95 sites (bands) are revealed, 27 of which are polymorplic, upon 28.42%; the Nei&Li's genetic similarity within population is 90.67% and Shannon's information index is 0.091 8 bit. In the second group, 46 sites(bands) are revealed, 12 of which are polymorphic, upon 26.09%; the Nei & Li's genetic similarity is 93.27% and Shannon's information index is 0.067 0 bit. The genetic diversity of Pelteabagrus fulvidraco is lower than that of reported Carassius auratus L. and Gobiocypris rarus and higher than Aristichthys nobilis .

关 键 词：黄颡鱼 RAPD标记 遗传多样性 Shannon信息指数 遗传标记 遗传相似率 

分 类 号：Q959.403[生物学—动物学]