不同细胞质雄性不育小麦中依赖钙/钙调素的蛋白激酶的SDS-PAGE电泳图谱分析  

The SDS-PAGE of Ca^(2+) /CaM-dependent Protein Kinase in Different Types of Cytoplasmic Male Sterile Wheat

作　　者：史红梅[1] 何之常[2] 胡德文[2] 徐乃瑜[2] 

机构地区：[1]华中农业大学理学系,湖北武汉430071 [2]武汉大学生命科学学院,湖北武汉430072

出　　处：《武汉大学学报（自然科学版）》2001年第2期243-246,共4页Journal of Wuhan University(Natural Science Edition)

基　　金：国家基础性研究攀登计划资助项目! (PD-XZ- -4 b)

摘　　要：以几种新型细胞质雄性不育型小麦为材料 ,对分蘖期小麦叶片中的依赖钙 /钙调素的蛋白激酶 (Ca2 + /Ca M- PK)的 SDS- PAGE电泳图谱进行了分析 ,实验结果表明 ,不育系与保持系的蛋白激酶酶带之间存在差别 ,并且不育系之间也有差别 ,说明不育细胞质对核基因的表达也有影响 ,这可能与雄性不育有关 ,而且不育材料中存在潜在优势 。Using four types(C,G,M,Mt 2) of cytoplasmic male sterile wheat as experiment materials, their maintainer Chinese spring wheat (T.aestivum) as contrast, the zymograms of Ca 2+ /CaM-dependent protein kinase were studied. The electrophoresis were carried out after crude enzyme of Ca 2+ /CaM-PK were extracted from leaves in tillering stage. The bands were well separated upon linear gradient SDS-PAGE(6%\|15%).There existed difference among male sterile materials, maintainer and Huamai No.8. So the cytoplasm have different effect on the expression of gene, which probably associated with the cause of male sterility.The new male sterile wheat may be used to create new hybrid.

关 键 词：依赖钙/钙调素 蛋白激酶 杂种优势 小麦 SDS-PAGE电泳图谱 细胞质雄性不育性 

分 类 号：S512.101[农业科学—作物学] Q946.569[生物学—植物学]