亚硒酸钠与细胞的作用  

Effects of Sodium Selenite on Cell

作　　者：温和瑞[1,2] 陈荣三[1,2] 

机构地区：[1]江西赣南师范学院化学系 [2]南京大学配位化学研究所

出　　处：《微量元素与健康研究》1998年第2期6-7,26,共3页Studies of Trace Elements and Health

摘　　要：通过测定巨噬细胞的存活率、脂质过氧化、细胞内游离Ｃａ２＋、Ｍｇ２＋的浓度和红细胞膜Ｃａ２＋－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶的活性，研究了亚硒酸钠与细胞的作用。结果表明高浓度的Ｎａ２ＳｅＯ３（≥１０－４ｍｏｌ·Ｌ－１）有明显的细胞毒性；Ｎａ２ＳｅＯ３对巨噬细胞的毒性作用引起细胞内游离Ｃａ２＋、Ｍｇ２＋浓度升高；但Ｍｇ２＋浓度升高比Ｃａ２＋慢；较高浓度的Ｎａ２ＳｅＯ３（≥１０－５ｍｏｌ·Ｌ－１）能抑制红细胞膜Ｃａ２＋－ＡＴＰ酶和Ｍｇ２＋－ＡＴＰ酶的活性，但对Ｍｇ２＋－ＡＴＰ酶活性的抑制作用要低于对Ｃａ２＋－ＡＴＰ酶活性的抑制作用。The effects of Na2SeO3 on macrophage were studied by means of the survival percentage of macrophage,the lipid peroxides,the cytoplasmic free Ca2+and Mg2+concentration,the activity of Ca2+-ATP ase and Mg2+-ATP ase of erythrocyte membrane.The results showed that the high concentration Na2SeO3(≥10-4mol·L-1)had the cytotoxicity.The poisonous effect of Na2SeO3 on macrophage led to the increase of cytoplasmic free Ca2+and Mg2+concentration,but the increase of cytoplasmic free Mg2 concentration was slower than that of cytoplasmic free Ca2+concentration.High concentration Na2SeO3 inhibited the Ca2+-ATPase and Mg2+-ATPase activity.But the inhibition to Mg2+-ATPase was Weaker than that to Ca2+-ATPase.

关 键 词：亚硒酸钠 细胞毒性 脂质过氧化 镁离子 CA^2+-ATP酶 Mg^2+-ATP酶 红细胞膜 钙离子 

分 类 号：Q581[生物学—生物化学] Q461