沈阳地区肺炎支原体分离株P1蛋白基因限制性酶切图谱分型研究  

Study on Endonuclease Map of P1 Protein Gene of Mycoplasma Pneumoniae Isolated Strain in Shenyang

作　　者：王桂珍[1] 董占双[1] 刘中顺[1] 李保强[2] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,沈阳110001 [2]中国人民解放军第202医院检验科

出　　处：《中国医科大学学报》2001年第2期103-104,共2页Journal of China Medical University

摘　　要：目的 :根据肺炎支原体 (MP)P1蛋白基因限制性酶切图谱分类MP临床分离株 ,调查沈阳地区肺炎支原体流行情况。方法 :多聚酶链反应扩增MPFH标准株和MP临床分离株的P1基因片段 ,扩增产物用限制性内切酶HeaIII消化 ,1.5 %琼脂糖凝胶电泳分析。结果 :肺炎支原体临床分离株和FH标准株酶切图谱相同。结论 :沈阳地区肺炎支原体分离株属于Ⅱ型。Objective: Our aims were to classify mycoplasma pneumoniae (MP) clinical isolated strain according to endonuclease map of the PCR products of the MP P1 protein gene and to investigate MP epidemic state in Shenyang.Methods:The P1 protein gene fragment of MP isolated strain was amplified by using PCR method ,and its products were digested with Hea III, 1.5 % argarose gel electrophoresis.Results:Isolated strain showed the same map as MP FH standard strain.Conclusion: Clinical isolated strain of mycoplasma pneumoniae in Shenyang belongs to groupⅡ.

关 键 词：肺炎支原体 P1基因 限制性内切酶图谱 分型 

分 类 号：R375.2[医药卫生—病原生物学]