神经生长因子对新生大鼠缺氧缺血性脑损伤的保护作用  

Protective Effects of Nerve Growth Factor on Hypoxic-ischemic Brain Damage in Neonatal Rats

作　　者：胡勇[1] 邬建荣[1] 孔元原[1] 俞海国[2] 汤云珍[2] 

机构地区：[1]贵阳医学院附院儿科,贵州贵阳550004 [2]东南大学附属中大医院儿科,江苏南京210000

出　　处：《中国当代儿科杂志》2001年第2期170-172,共3页Chinese Journal of Contemporary Pediatrics

摘　　要：目的　研究神经生长因子 (NGF)对新生大鼠缺氧缺血性脑损伤 (HIBD)的保护作用。方法　将新生 7日龄SD大鼠 40只随机分为NGF治疗组 (n =16 ) ,对照组 (n =16 )和假手术组 (n =8) ,缺氧缺血 (HI)后即刻腹腔注射 10 0UNGF或等量生理盐水 (假手术组不注射 ) ,然后观察NGF对HIBD模型鼠的体重增长、脑组织病理及超微结构改变的影响 ,并用TUNEL法原位标记DNA片段 ,观察NGF对HIBD后脑细胞凋亡的影响。结果　NGF治疗组体重增长 (4 .16± 0 .2 4)g明显高于对照组 (2 .86± 0 .17)g ,(P <0 .0 1) ;TUNEL检测结果 ,HIBD后 2 4h治疗组左侧海马和皮质凋亡细胞数 (分别为 199.75± 19.6 1,182 .75± 19.12 )明显低于对照组 (分别为 2 85 .5 0±32 .6 7,2 71.0 0± 2 8.36 ) (P <0 .0 1) ;HIBD后 48h治疗组左侧海马、皮质凋亡细胞数 (分别为 77.75± 15 .76 ,82 .5 0± 19.15 )亦明显低于对照组 (分别为 10 6 .5 0± 16 .96 ,12 2 .75± 16 .5 6 ) (P <0 .0 1)。Objective To explore the protective effects of nerve growth factor (NGF) on hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods Forty 7 day postnatal rats were randomly divided into NGF treated (n=16), control (n=16) and sham surgery groups (n=8). Immediately after hypoxic ischemic (HI) injury, 100 U NGF or normal saline solution was injected intraperitoneally; the sham surgery group was not injected. The effects on body weights, macro and microscopical changes were then assessed. The effect on apoptosis of neurons was observed by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling (TUNEL) staining. Results The increased body weight in the NGF treated group was significantly higher than that in the control group [( 4.16 ± 0.24 ) g and ( 2.86 ± 0.17 ) g, respectively (P< 0.01 )]. The average number of positive cells in the left hippocampus and cortex in the NGF treated group at 24 h after HIBD were much lower than those in the control group ( 199.75 ± 19.61 vs 285.50 ± 32.67 , 182.75 ± 19.12 vs 271.00 ± 28.36 , respectively, P< 0.01 ). At 48 h after HIBD, they were also much lower than those in the control group ( 77.75 ± 15.76 vs 106.50 ± 16.96 ; 82.50 ± 19.15 vs 122.75 ± 16.56 , respectively, P< 0.01 ). Conclusions It is suggested that intraperitoneal administration on NGF has protective effects on neuronal apoptosis associated with hypoxic ischemic injury.

关 键 词：缺氧缺血性脑损伤 神经生长因子 新生儿 细胞调亡 保护作用 

分 类 号：R722.12[医药卫生—儿科]