HPLC测定息痛宁胶囊中秦皮甲素和秦皮乙素的含量  

DETERMINATION OF AESCULIN AND AESCULETIN IN XITONGNING CAPSULE BY HPLC

作　　者：冉兰[1] 曾蓉[1] 晁若冰[1] 吴静[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2001年第2期135-136,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的 :测定中药复方制剂息痛宁胶囊中有效成份秦皮甲素和秦皮乙素的含量。方法 :采用HPLC ,ODS柱 ,流动相为甲醇 水 ( 2 4∶76 ) ,检测波长 336nm。结果 :秦皮甲素进样量在 0 0 0 5～ 1 5 μg范围内 ,峰面积与进样量有良好的线性关系 ,回收率 99 9% ;秦皮乙素进样量在 0 0 0 2 5～ 0 75 μg范围内 ,峰面积与进样量有良好的线性关系 ,回收率 10 2 7%。结论 :该方法准确性高 ,操作简单 。OBJECTIVE:An HPLC was used for determination of aesculin and aesculetion in Xitongning capsule.METHODS:Using HPLC with ODS column and methanol water(24∶76) pH 3.0 as mobile phase.Samples were detected at 336 nm.RESULTS:Linear ranges were 0.005～1.5 μg( r =1.0000)for aesculin and 0.0025～0.7 μg( r =0.9999)for aesculetin.The recoveries were 99.9%( RSD =1.7%) for aesculin and 102.7%( RSD =1.1%)for aesculetin.CONCLUSION:The method was simple and accurate and can be used to control the quality of this preparation.

关 键 词：秦皮甲素 秦皮乙素 息痛宁胶囊 高效液相色谱法 含量测定 

分 类 号：R971.1[医药卫生—药品] R927.2[医药卫生—药学]