TLC和HPLC法鉴别两种小粒菟丝子  

Identification of Seeds of Cuscuta australis and C. chinensis by TLC and HPLC

作　　者：叶敏[1] 周平[1] 阎玉凝[1] 黎艳[2] 刘虎威[2] 

机构地区：[1]北京中医药大学,100029 [2]北京大学化学系,100871

出　　处：《中药材》2001年第2期97-99,共3页Journal of Chinese Medicinal Materials

基　　金：国家九五攻关计划资助课题(NO.96-903-02-02)

摘　　要：采用TLC和HPLC两种方法鉴别菟丝子(Cuscuta.chinensis Lam.)和南方菟丝子(C.australis R.Br.)。TLC采用聚酰胺薄层层析,MeOH-HOAc-H_2O和CHCl_3-MeOH-HOAc两种溶剂二次展开。HPLC采用Hypersil-ODS柱,MeOH-0.025mol·L^(-1)H_3PO_4为流动相,流速1.0ml·min^(-1),检测波长360nm,柱温40℃。两种方法鉴别特征均很显著,且操作简便,重现性好。Idenfitication of seeds of Cuscuta australis R.Br.and C.chinensis Lam.was carried out by TLC and HPLC.Polyamide membrane was used as stationary phase,MeOH-HOAc-H2O and CHCl3-MeOH-HOAc were used as mobile phase for TLC.For HPLC,Hypersil-ODS column was used; the mobile phase was MeOH-0.025 M H3PO4; the flow rate was 1.0 ml·min-t; detection wavelength was 360 nm; and column temperature was 40℃.Both methods represented significant identification characteristics,and were simple,accurate and reproducible.

关 键 词：菟丝子 南方菟丝子 TLC HPLC 品种鉴别 高效液相色谱法 

分 类 号：R282.71[医药卫生—中药学]