四膜虫种间骨架蛋白组分的比较研究  

STUDIES ON CORTICAL CYTOSKELETAL PROTEINS OF DIFFERENT SPECIES OF TETRAHYMENA

作　　者：庞延斌[1] 孙卫华[1] 李书强[1] 

机构地区：[1]华东师范大学生物系,上海200062

出　　处：《水生生物学报》2001年第3期241-244,共4页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金!资助项目 ;编号 3 9870 0 94

摘　　要：以上海四膜虫S1 和嗜热四膜虫BF株和BT株为材料 ,结合显微观察 ,采用生化抽提、SDS -PAGE电泳、扫描及数据统计 ,分析与测定了三个不同株四膜虫对数生长期皮层骨架蛋白组分与含量 ,结果显示嗜热四膜虫的BF与BT株差异较小 ,两者与上海四膜虫S1 株差异则较大 ,S1 株细胞中有 92KD、72KD、66KD、32KD、2 7KD ,而BF和BT株细胞中没有 ,估计这些蛋白的不同与种间亲缘关系及株系。Cortical cytoskeletal proteins had been prepared by biochemical extraction with Tritonx-100, SDS-PAGE and scan, in order to study their difference of Tetrahymena thermophila BT, Tetrahymena thermophila BF and Tetrahymena shanghaiensis S 1. The data statistics had been analyzed by a computer. The results showed that the difference between T. thermophila BT and T. thermophila BF was little. The T. shanghaiensis S 1 had more protein patterns than those of two strains of T. thermophila . These proteins were 92KD, 72KD, 66KD, 32KD and 27KD. So the difference between Shanghaiensis S 1 thermophila and the two strains of T. thermophila was greater. We think that it was because of the difference of cultive medium and conditions that affected the components and contents of proteins.

关 键 词：嗜热四膜虫 上海四膜虫 皮层细胞骨架蛋白 SDS-聚丙烯酰胺凝胶电泳 

分 类 号：Q959.116[生物学—动物学]