中华鳖4个Sox基因保守区的序列分析  

THE CONSERVATIVE REGION SEQUENCE ANALYSIS OF FOUR Sox GENES IN THE TRIONYX SINENSIS

作　　者：聂刘旺[1] 单祥年[2] 汪鸣[1] 郭超文[1] 鲁晓萱[2] 

机构地区：[1]安徽师范大学生物系,芜湖241000 [2]南京铁道医学院铁道部分子生物学开放实验室,南京210097

出　　处：《水生生物学报》2001年第3期245-250,共6页Acta Hydrobiologica Sinica

基　　金：安徽省自然科学基金;江苏省自然科学基金! (项目编号 :974 1 2 0 0 2和 9750 61 1 2 )

摘　　要：采用PCR技术 ,扩增和克隆了中华鳖Sox基因 (TSSox)。经DNA序列分析显示 ,Sox基因在系统进化上十分保守 ,其中TSSox4与鸟类LF4基因编码的氨基酸序列完全相同、与人类SOX4和小鼠Sox4编码的序列仅一个氨基酸的差异 ;TSSox5与鸟类的LF5基因的编码也仅一个氨基酸发生了改变 ;TSSox2与海龟的TSox2相似性最高。 4条TSSox序列中 ,TSSox与人SRY基因序列相似性最高 ,达 75% ;The Sox genes of Trionyx sinensis were amplified and cloned using PCR. The four different fragments of Sox gene HMG-box DNA were selected and sequenced. The results indicated that the Sox gene is conservative in phylogeny, in which the amino acid sequence encode by TSSox4 (The Sox of T. Sinensis ) is identical to those encode by LF4 (bird) or differ from SOX4(human) and Sox4(mouse) only on one amino acid; The amino acid sequences encode by TSSox5 have an amino acid difference from those encode by LF5(bird); TSSox1 has about 75% identical to SRY HMG-box at the nucleotide level, and is most resemblance among four TSSox. Phylogenetic analysis of available 32 Sox sequences including SRY/Sry sequence suggests that there wolud be a high degree of divergence between any possible immediate common ancestor of the T. Sinensis Sox sequence and the SRY(Sry) sequences.

关 键 词：中华鳖 SOX基因 序列分析 PCR 保守区 性别决定 

分 类 号：Q959.63[生物学—动物学] Q344.2