应用5.8S rDNA及ITS区序列分析链格孢种级分类  

APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL

作　　者：王洪凯[1] 张天宇[2] 张猛[3] 

机构地区：[1]中国科学院真菌地衣系统学开放实验室,北京100080 [2]山东农业大学植病系,山东泰安271018 [3]浙江大学生物技术研究所,杭州310029

出　　处：《菌物系统》2001年第2期168-173,共6页Mycosystema

基　　金：国家自然科学基金资助项目!(项目号:39770003)&&

摘　　要：对9个链格孢小孢子种和3个大孢子种共20个链格孢菌株的5.8S rDNA及其两侧的ITS1区和ITS2区进行了序列分析。聚类分析结果表明形态差异大的种可以明确加以区分，而供试的9个小孢子种之间差异很小，不能根据对所选区段的序列分析加以区分。传统分类上的滨菊链格孢不属于Alternaria, 其分类地位需进一步研究。： The sequences coding for the 5.8S rDNA and the flanking internal transcribed spacers (ITS1 and ITS2) were amplified by PCR and sequenced from 9 small-spored species and other 3 large-species in Alternaria. Phylogenetic analysis clearly separated the 2 large-spored species from each other and from 9 small-spored species tested, but small-spore species cannot be clearly separated each other. This indicate that small-spored species tested belong to a natural species group. Traditional taxonomic morphologically distinguished species A.leucanthemi may not belong to Alternaria according to sequence analysis, its taxonomic position require further study.

关 键 词：链格孢 序列分析 ITS区 rDNA5.8s段 分类地位 

分 类 号：Q949.32[生物学—植物学]