从实验感染动物粪便中分离出两株疑似戊型肝炎病毒  

Isolation of Two Strains of Suspected Hepatitis E Virus from Infected Experimental Animal Stools

作　　者：陈万荣[1] 田辛[1] 鲍作义[1] 殷书荣[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国病毒学》1993年第1期111-113,共3页Virologica Sinica

摘　　要：用Hep-G_2细胞从肠道传播的非甲非乙型肝炎(ET-NANBH)病人粪便悬液感染恒河猴的潜伏期粪便中分离出两株致细胞病变(CPE)的病毒(R_(19),R_(25))。用免疫电镜(IEM)观察,这两株病毒均为球形,有实心和空心两种颗粒,直径约27—34nm,能被新疆和前苏联ET-NANBH急性期血清特异性凝集,病毒颗粒间抗体桥明显,ET-NANBH急性期血清对这两株病毒亦有一定中和作用,内地甲肝(HA)病人和正常人血清既不能凝集,也不能中和,是否为ET-NANBH病原体尚待进一步鉴定。The Hep-G_2 cell lines were inoculated for isolating HEV and two strain CPE Viruses (R19, R25)were isolated from experimental infected monkey's acute phase fecal. IEM revealed that the isolated two strain viruses could be aggregated by acute phase sera of ET-NANBH patients from Xinjiang and USSR respectively and there were obvious antibodies and full, spheric 1 27-34nm virus-like empty particles (VLPS). The two strain viruses could be neutralized by acute phase sera of HE patients. The sera of patients from inland area and of normal people could not aggregate and neutralize the two strain viruses, Whether the two strain viruses are HE etiologic ag ents should be investigated further.

关 键 词：戊型肝炎病毒 实验动物感染 组织培养 病毒分离 

分 类 号：R373.21[医药卫生—病原生物学]