抗EHFV的独特型抗体在小鼠体内诱导免疫应答的研究  

The Production of ldiotypic Antibody against EHFV and Immunoreaction of Anti-EHFV Induced by the Idotypic Antibody in Mouse

作　　者：李川江[1] 林乔[1] 王懋梁 

机构地区：[1]湖北省医学科学院病毒研究所,武汉430070

出　　处：《中国病毒学》1991年第4期309-314,共6页Virologica Sinica

摘　　要：本文介绍用流行性出血热(EHF)病毒J10株制备单克隆抗体(McAb),以及用7个McAb免疫家兔,使其产生抗EHF病毒McAb的抗体即抗独特型抗体(Ab_2)。Ab_2能在体外同出血热病人恢复期血清和EHF病毒免疫的兔血清发生特异性结合。再经EHF-McAb亲和层析法分离提纯Ab_2,免疫BALb/c小鼠,将所获得的免疫血清(Ab_3)用荧光和ELISA分别加以测定,结果表明,抗-抗独特型抗体可在体外识别EHF病毒,而不能同病病人恢复期血清发生结合,从而支持免疫网络学说,可能为我们提供一种新型的抗原来源途径。In this papar, the preparation of menoclonal antibodies using epidemic hemorrhagic fever virus J10 strain and anti-EHFV McAb antibody (idiotypic antibedy, Ab2)prcduced by rabbt immunized with 7 clones of McAb are described In vitro, Ab2 was able to combine specifically with serum of recovering patients with EHF and serum of rabbit immunized by EHFV. Ab2 separated and purified by means of affinity chromatography was used to immunize Balb/c mice. The immunized serum (Ab3) was determined by immunoflouresence and ELISA. The result indicated that, in vitro, anti-antiidiotypic antibody could identify EHFV but could not combine with serum of recovering patients with EHF, which supports the theory of immunonetwork and will probably provide a way to obtain new antigen.

关 键 词：流行性出血热病毒 单克隆抗体 独特型抗体 抗独特型抗体 免疫应答 

分 类 号：R373.32[医药卫生—病原生物学]