转pGH基因猪外源基因染色体定位研究  

Exogenous pGH Gene Localization on Chromosomes of the Transgenic Pigs

作　　者：牛屹东[1] 何新[2] 陈清轩[2] 林爱星[3] 陈永福[3] 魏庆信 

机构地区：[1]中国科学院动物研究所 [2]中国科学院发育生物学研究所,北京100080 [3]中国农业大学生物学院,北京100094 [4]湖北省农科院畜牧研究所,武汉430209

出　　处：《生物工程学报》2001年第3期318-321,共4页Chinese Journal of Biotechnology

基　　金：国家"8 6 3"项目! ( 86 3 10 1 0 5 0 1);"973"项目部分资助! (G2 0 0 0 16 10 7)&&

摘　　要：应用地高辛标记探针对转pGH基因 (猪生长激素基因 )猪染色体进行原位杂交 ,经胶体金抗体、银增强放大系统检测外源pGH基因在染色体上的整合位点。研究表明 ,转基因阳性个体间外源基因整合位点存在差异 ,但对个体而言 ,外源基因总是集中分布于某一特定的染色体上。The pUC19\|OMT plasmids were cut by \%Ssp\%1 and the products in 900bp were recovered from low\|melting agarose gel and used as probes which were labeled by Digoxigenin.After being denatured,the probes were dropped on the chromosome samples which were also denatured to anneal with them.The anti\|digoxigenin antibodies labeled with colloidal golds were used to act with the chromosome samples.In order to localize the exogenous pGH genes(porcine growth hormone gene) on chromosomes detected with optical microscope and improve the sensitivity,digoxigen gold signals are amplified by silver precipitation.After calculating the number of silver grains on every chromosome under the optical microscope,we analyzed the data with statistical methods.The results show that the integrating sites of exogenous pGH genes are very different among the positives.However,it is clear that the exogenous genes in one are always of the tendency to integrate in one specific site on a certain chromosome.These data are of great significance for studying the site\|specific integration and the expression efficiency of exogenous genes in the future research.

关 键 词：pGH基因 转基因猪 染色体原位杂交 基因定位 

分 类 号：Q75[生物学—分子生物学]