日本对虾血清三类免疫球蛋白样物质的研究  

STUDY ON THREE CLASSES OF IMMUNOGLOBULIN-LIKE COMPONENTS IN Penaeus japonicus

作　　者：章跃陵[1] 彭宣宪[1] 王三英[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室361005

出　　处：《海洋科学》2001年第5期37-41,共5页Marine Sciences

基　　金：国家海洋局第三海洋研究所海洋生物工程重点实验室开放课题基金!资助项目

摘　　要：采用单向免疫扩散法 (SRID)和免疫酶斑点法 (Dot ELISA)对日本对虾 (Penaeusjaponicus)血清中类IgM ,IgG ,IgA样物质进行了较系统的研究分析。结果发现 ,虾血清中存在能与羊抗人IgM ,IgG ,IgA发生结合且能被特异性阻断试验确证的物质。文中还对12例对虾血清类IgM ,IgG ,IgA样物质的含量进行了检测。结果提示 ,虾血清中确实存在与人免疫球蛋白 (Ig)特异性抗原决定簇极为相似的物质 ,它的存在对研究虾的抗病机制具有重要意义。Both methods of single radial immunodiffusion(SRID) and dot ElISA were used to characterize three kinds of immunoglobulin like proteins (IgM, IgG and IgA) in serum of Penaeus japonicus. The results showed that there were components reacted with anti human μ,γ or αchain in the serum, which could be blocked by specific tests. Furthermore, the IgM like, IgG like and IgA like components were measured in 12 cases of Penaeus japonicus. These findings suggest that there were materials with similar isotopes in serum from both the shrimp and human. And Ig like proteins in the shrimps were important to understand their disease free mechanism.

关 键 词：日本对虾 免疫球蛋白 单向免疫扩散 免疫酶斑点法 血清 抗病机制 

分 类 号：Q959.223[生物学—动物学]