血栓闭塞性脉管炎与人巨细胞病毒感染的相关性  

The relationship between human cytomegalovirus infection and thromboangiitis obliterans

作　　者：陈以宽[1] 朱仕钦[1] 高根五[1] 常正[1] 李生伟[1] 孙建民[1] 田泽丹[1] 

机构地区：[1]重庆医科大学附属第二医院普外科,400010

出　　处：《中华普通外科杂志》2001年第5期272-273,共2页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目!(395 70 6 92 )

摘　　要：目的　考查血栓闭塞性脉管炎血管壁中人巨细胞病毒的感染情况。方法　以人巨细胞病毒即刻早期 (IE)基因上游的增强子为扩增区段 ,用PCR技术分析了 41例血栓闭塞性脉管炎病变血管壁中和 14例正常血管壁中人巨细胞病毒的感染情况。RCR产物电泳后观察结果。结果　 41例血栓闭塞性脉管炎病变血管壁中有 2 8例 (6 8% )查到人巨细胞病毒DNA的存在 ,14例正常血管壁中有 2例 (14% )查到人巨细胞病毒DNA的存在。结论　在血栓闭塞性脉管炎病变血管壁中存在人巨细胞病毒的感染 。Objective [WT5”BZ] To investigate human cytomegalovirus infection (CI) within the vascular wall of thromboangiitis obliterans (TO).[WT5”HZ] Methods [WT5”BZ] Vascular specimens from 41 patients with thromboangiitis obliterans and 14 normal controls were analyzed by PCR. The nucleic acid sequence of enhancer located in the upstream of immediate early(IE) gene of cytomegalovirus was used as target region for detection of cytomegalovirus DNA. The PCR products were visualized after electrophoresis. [WT5”HZ]Results [WT5”BZ] Cytomegalovirus DNA was found in 28 (68%) out of the 41 specimens and only in 2 (14%) out of the 14 normal vessels. [WT5”HZ]Conclusions [WT5”BZ] The results suggested that there was an evidence of CI in the vascular wall of TO. The association between CI and TO needs further investigation.

关 键 词：巨细胞病毒属 血栓闭塞性脉管炎 聚合酶链反应 

分 类 号：R654.4[医药卫生—外科学]