rGH+CLS减少电损伤组织坏死的研究  

Reducing Tissue Necrosis in Electrical Injuries with rGH CLS

作　　者：许晓光[1] 李伟萍[1] 刘天一[1] 朱志祥[1] 吴金浪[2] 

机构地区：[1]深圳市红十字会医院烧伤整形科,深圳518035 [2]中山医科大学电镜室,广州510200

出　　处：《昆明医学院学报》2001年第2期25-28,共4页Journal of Kunming Medical College

基　　金：广东省卫生科研基金 (A - 19995 47);卫生部基金 (1996 - 188- 2 70 )资助项目

摘　　要：研究rGH +CLS局部应用对减少高压电损伤组织进行性坏死的作用 ,使用该实验室已建立的重度高压电损伤模型 .新西兰大白兔 45只均分 5组 ,治疗分别使用rGH +CLS (1组 ) ,CLS (2组 ) ,rGH (3组 ) ,盐水 (4组 )和 5组对照 .临床解剖探查、组织学和超微结构等方法观察 .结果 :治疗 1组有明显的减少组织坏死作用 ,15d动物伤肢完整 .而 2 ,3和 4组虽也各有不同程度延缓坏死的作用 ,但动物伤肢最终坏死丧失 ,5组 7d大部软组织基本坏死 ,12d坏死离断 .光电镜检查 1组 5d治疗后粒细胞浸润较少 ,炎性反应轻 ,有纤维组织细胞再生和新生的线粒体 ,10d修复过程明显 ,2 ,3,4组 7d后坏死明显 ,5组肌坏死迅速 .结果表明 :CLS持续组织内透析冲洗和rGH局部肌注能有效地减少电损伤坏死速度和范围 ,值得研究应用 .To study the curative effect of rGH for electrical injury wounds, 45 rabbits of severe electrical injury were divided into 5 groups treated with rGH and CLS( Chloromyc etin 1?000?mg, Lidocain 1?000?mg, and Salin 500?mL) in group 1, CLS in group 2, rGH in group 3, saline in group 4, and control in group 5, and that was investigated using clinical vivisection, histology and ultrastructures. Results: Group 1 obtained an excellent curative effect and limbs was restored with wound limited within section ABC in 15 days following injury, in contrast with those limbs in groups 2～4 necrosed after 8 days and lost in less than 14 days following injury, and tissue necrosis in group 5 was developing rapidly and limbs lost in 12 days following injury. Histology and ultrastructures: Slight inflammatory reaction with rarely granular leukocytes infiltration and neogenesis mitochondrions and fibrous cells were observed in 5 days following in jury and renovation process in muscle structures developed in 10 days after injury in group 1; muscle fibers dissolution necrosed in group 2,3,4 after 5 days following injury, and more serious dissolution appeared in group 5. Conclusion: The curative effects on reducing the tissue necrosis with CLS dialysis and rGH injected in local site is remarkable and worthy of further study and application.

关 键 词：电损伤 组织进行性坏死 CLS RGH 

分 类 号：R647[医药卫生—外科学]