用DNA探针检测沙眼衣原体  

Detection of Chlamydia Trachomatis by DNA Probe Blot

作　　者：王卫萍[1] 陈亚利[1] 武建国[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,江苏南京210002

出　　处：《中华男科学杂志》2001年第2期102-104,共3页National Journal of Andrology

摘　　要：目的 :建立一种敏感而特异的沙眼衣原体分子生物学检测方法。　方法 :用PCR扩增 5 17bp的沙眼衣原体种特异性基因片段并标记成探针 ,建立DNA探针杂交检测沙眼衣原体的方法。　结果 :探针只与沙眼衣原体L2、TE5 5株DNA呈阳性杂交斑点 ,与其他两种衣原体、解脲支原体、淋病奈瑟菌、大肠埃希菌、金黄色葡萄球菌、流感嗜血杆菌及白色念珠菌DNA斑点膜无阳性杂交信号。从 10 0例慢性宫颈炎和前列腺炎病人生殖道分泌物中检出阳性 2 2例 ,阳性率 2 2 %。　结论 :建立的DNA探针检测沙眼衣原体方法具有较高的敏感性和特异性 。Objectives: To establish a sensitive and specific molecular biological method for detecting Chlamydia trachomatis of genital tract infection patients. Methods: A DNA blot assay was developed by coating DNA of Chlamydia trachomatis and/or extract of clinical samples on nitrocellulose (NC) membrane, blotting with a DNA probe labeled with DIG. Results: There was no positive blotting in Chlamydia pneumoniae , Chlamydia psittaci , Ureaplasma urealyticum , Neisseria gonorrhoeae , Esherichia coli , Staphylococcus aureus , Haemophilus infuenzae and Candida albicans except Chlamydia trachomatis . The sensitivity could be improved to 1pg. The positive percentage was 22% (22/100) in detection of swabs collected from 100 chronic cervicitis and prostatitis patients. Conclusions: This method was not only sensitive, rapid and specific but also could be applied to detect batch samples.

关 键 词：DNA探针 沙眼衣原体 斑点杂交 

分 类 号：R446.5[医药卫生—诊断学]