延衰中药补肾益精方对大鼠左心室α、βMHCmRNA增龄变化的影响  

Effect of Retarding Aging Chinese Drug Bushen Yijing Decoction on Rat Left Ventricular Myosin Heavy Chain (MHC) mRNA Change with Aging

作　　者：童普德[1] 王晓玲[1] 胡旭东[1] 林水淼 蒋文娟[3] 李伯勤[3] 

机构地区：[1]上海中医药大学生物教研室,上海200032 [2]上海中医药研究院老年所,上海200032 [3]上海中医药大学机能实验室,上海200032

出　　处：《中国中医基础医学杂志》2001年第4期34-36,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：上海市教委资助课题! (96CJ0 3)

摘　　要：目的 :研究延衰中药补肾益精方对大鼠衰老过程 (8～ 2 4月龄 )中左心室肌球蛋白重链 (α、βMHC)基因转录mRNA表达量的影响 ,进一步探讨补肾益方的延衰机理。方法 :大鼠喂饲到 18月龄时随机分成老年组和老年服药组 ,中药用量是成人用量的 30倍 ,服药 6个月。以 8月龄大鼠作为成年对照组。大鼠处死后 ,以常规放免法测定血清甲状腺素T3 和T4 水平。用RT PCR方法扩增三组动物左心室α、βMHC基因的有关转录产物 ,以GAPDH扩增片段做相对定量。结果 :老年服药组与老年组相比 ,T4 增加到 1 2 4倍 (P <0 0 0 1) ,αMHCmRNA增加到 1 39倍 (P <0 0 5) ,βMHCmRNA下调到 74 32 % (P <0 0 5)。结论 :延衰中药对大鼠衰老增龄中α、βMHC基因表达的衰老变化有逆转作用。Aim:To investigate the effect of BuShen Yi Jing decoction on rat left ventricular myosin heavy chain(MHC)α and βgene expression change with aging. Methods:Wistar rats were randomly divided into three groups:untreated 8 and 24 months of aging,administration drug 24 months of aging. Rats were administration buShen Yi Jing decoction six months with 30 times adults human dose. Rats serum thyroxine(T 4) and triiodothyronine(T 3) levels using an radioimmunoassay(RIA) from clinic assay,RT-PCR method was used to evaluate α and βMHC mRNA expression. Results: T 4 level was increased 1.24 times of senescent administration drug rats contrast to senility control rats ( P <0.001),αMHC mRNA enhanced 1.3 times ( P< 0 05) and β MHC mRNA decreased 74.32%( P <0.05). Conclusion: Bushen Yijing decoction had reversed effect on α and βMHC gene expression change with aging.

关 键 词：补肾益精方 肌球蛋白重链 mRNA 甲状腺素 RT-PCR 中药 药理 

分 类 号：R285.5[医药卫生—中药学]