降糖丸中葛根素的薄层扫描测定  

Determination of Puerarin in Jiangtang Pill by TLC

作　　者：许英爱[1] 王雅君[1] 李砥辉[1] 

机构地区：[1]第四军医大学吉林军医学院,吉林市132013

出　　处：《中国药房》2001年第5期299-300,共2页China Pharmacy

摘　　要：目的 :建立薄层扫描法测定降糖丸中葛根素含量的方法。方法 :固定相为硅胶GF254,流动相为氯仿 -甲醇 -水 (7∶2 5∶0 25) ,检测波长为254nm ,参比波长为370nm。结果 :葛根素在0 4μg～2 0μg 范围内呈良好的线性关系 ,平均回收率为99 4 %(n=5) ,RSD为1 37 %。结论 :本方法准确、可靠 ,可用于测定降糖丸中葛根素的含量。OBJECTIVE:To develop a method of thin-layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS:Silica GF254 thin-layer was used,and the mobile phase consisted of chloroform-methanol-water(7∶2 5∶0 25) with the detection wave-length of 254nm and the control wave-length of 370nm RESULTS:The linear range of puerarin was 0 4～2 0μg The mean recovery was 99 40%(n=5) with RSD of 1 37% CONCLUSION:This method is accurate,reliable and can be used for the determination of puerarin in Jiangtang pill

关 键 词：薄层扫描法 葛根素 降糖丸 中药 

分 类 号：R284.1[医药卫生—中药学]