毛白杨无性系组培芽分化效应与离体选种的研究  

RESPONSE OF ADVENTITIOUS BUDS FORMATION AND CLONE SELECTION IN VITRO FOR POPUWS TOMENTOSA

作　　者：袁巧平[1] 董茂山[1] 黄钦才[1] 刘颂 顾万春[1] 

机构地区：[1]中国林业科学研究院林业研究所

出　　处：《林业科学研究》1990年第6期608-612,共5页Forest Research

摘　　要：毛白杨(Populus tomentosa)是我国华北地区的重要用材和绿化树种。从70年代开始,采用常规方法经无性系大田多点对比试验,已选出一些优良无性系,并进行了组培繁殖。离体培养条件下毛白杨不同无性系的器官分化能力存在着明显差异。本文采用8个毛白杨无性系为材料,在分析离体组织的生长分化特性与植株大田生长特性的关系的基础上,探索毛白杨生长性状的试管微型选择的可能性,并找出各无性系的最佳分化培养基,提高组培繁殖效率。Adventitious buds formation was tested in 21 media with shoot segments of 8 clones of Populus tomentosa. Considerable variation was found in the response of adventitious buds formation among clones. Regression analysis showed a highly significant correlation existed between clonal response of adventitious buds formation and clonal growth response (r = 0.86**, a = 0.01). This suggests clone selection in vitro for growth is possible. Clonal response of adventitious buds formation was also significantly correlated with the contents of some endogeous hormones and amino acids (r= 0.70~ 0.76*, a=0.02). While negative correlation occured between clonal response of adventitious buds formation and clonal rooting ability of cuttings in fields. Composition of medium strongly affected the behavior of cultures.

关 键 词：毛白杨 组织培养 离体选种 不定芽 分化效应 优良无性系 

分 类 号：S792.117[农业科学—林木遗传育种]