脐血造血细胞体外扩增及其粘附特性变化  

STUDY ON HUMAN UMBILICAL CORD BLOOD HEMOPOIETIC CELLS EXPANDED IN VITRO AND ADHESIVE CHARACTERISTIC

作　　者：常英军[1] 邹正辉[2] 曹祥山[2] 梁建英[2] 

机构地区：[1]河北医科大学第三医院血液科 [2]苏州大学附属第二医院血液科,苏州215004

出　　处：《河北医科大学学报》2001年第3期137-139,共3页Journal of Hebei Medical University

摘　　要：目的探讨脐血造血细胞体外扩增过程中的粘附特性变化 ,为扩增最佳时机的选择提供实验依据。方法用长期培养法体外扩增脐血造血细胞 ,并用流式细胞仪测定其表面粘附分子变化。结果CD11b表达于第 10天显著增加 ,第 30天达 34.3 % ;CD44表达于第 2 0天达最高水平 ;CD5 4表达则于第 5天明显增加 ,第 30天达14.9% ;CD34+造血细胞在第 15天达高峰 ,之后渐下降 ,并维持在一定水平。CD44及CD5 4表达与CD34+造血细胞变化存在相关性。结论脐血造血细胞体外扩增过程中 ,CD44和CD5 4的持续高表达有利于造血细胞移植后的归巢 ;移植的最佳时机以长期培养第 14天为宜。ObjectiveTo investigate the variation of cell adhesion molecules (CAMs) on umbilical cord blood (UCB) hemopoietic cells (HPCs) expanded in vitro and the optimal time for transplantation.MethodsLong term culture (LTC) was used to expand the UCB HPCs.The expression of CAMs were determined by flow cytometry.ResultsCD11b + cells increased significiantly at the 10th day, and quickly increased to 34.3 % at the 30th day.CD54 + cells increased greatly at the 5th day, and reached 14.9 % at the 30th day. CD44 + cells reached higher level at the 20th day, while CD34 + cells reached highest level at the 15th day, then decreased gradually but maintained at a higher level than that at the 5th day. Linear regression analysis showed there were interrelated between the expression of CD54 or CD44 and CD34 + HPCs.ConclusionThe expression of CD54 and CD44 on UCB HPCs could play a positive role in the homing of UCB HPCs after its transplantation, the suitable time for transplantation is at the 14th day.

关 键 词：胎血 细胞学 造血干细胞移植 细胞培养 体外扩增 粘附特性 

分 类 号：R331.2[医药卫生—人体生理学] R457.7[医药卫生—基础医学]