球囊血管成形术后局部PAI-1基因的表达  

Changes of Platelet, Fibrinolytic Activity and Expression of PAI-1 Gene after Balloon Injury in Rabbits

作　　者：陈晓文[1] 戚文航[1] 

机构地区：[1]上海第二医科大学瑞金医院心内科,上海200025

出　　处：《上海第二医科大学学报》2001年第3期220-222,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的　建立兔再狭窄模型，系统观察球囊血管成形术后血浆Ｐ－选择素含量，纤溶酶原激活物抑制剂－１（ＰＡＩ－１）活性的变化，ＰＡＩ－１基因表达，以了解ＰＡＩ－１基因表达与再狭窄间关系。方法　对模型兔进行球囊血管成形术，分别于术前、术后３ｈ，１、２和４周应用ＥＬＩＳＡ法测定血浆Ｐ－选择素含量，应用发色底物法测定血浆ＰＡＩ－１活性，并利用ＲＴ－ＰＣＲ技术测定不同时期局部血管壁内ＰＡＩ－１ｍＲＮＡ表达情况。结果　血管成形术后３ｈ，血浆Ｐ－选择素含量、ＰＡＩ－１活性均迅速升高，血管局部ＰＡＩ－１ｍＲＮＡ在术后３ｈ明显增加达高峰，术后４周达术前水平。结论　血管成形术后，血小板活化，局部ＰＡＩ－１表达增加，不仅促使损伤局部血栓形成，而且还可刺激局部血管平滑肌增生，并通过抑制局部纤溶活性而促使细胞外基质堆积，在再狭窄形成中起重要作用。Objective To demonstrate possible association between changes in platelet activity, fibrinolytic potential and restenosis in the balloon - injured rabbit model, following observation on the changes of plasma P - selectin, plasma plasminogen activator inhibitor type 1(PAI - 1 ) activity and the expression of PAI - 1 gene in the arterial wall after vascular injury. Methods In the rabbit model subjected to balloon injury, plasma PAI - 1 activity was determined by chromogenic assay; P - selectin was assayed by ELISA, and PAI - 1 messenger ribonucleic acid (mRNA) expression was assayed with the use of RT - PCR. Results The plasma level of P - selectin increased rapidly from 7.82 ± 0.36ng/ml to 48.43 ± 13.51ng/ml at 3h after balloon injury. PAI - 1 activity in plasma increased sig- nificantly from 7.90 ± 0.87AU/ml to 11.83 ± 1.05AU/ml at 3h after vascular injury. The expression of PAI - 1 mRNA was increased 3.4 - fold compared with that in the control arteries at 3h after vascular injury. Conclusion Our results indicate that balloon injury simulating angioplasty induces high - ac- tivity of the platelet and intramural expression of PAI - 1. The decreased cell surface fibrinolytic activity is likely to result from the increased PAI - 1 expression which may initiate or exacerbate mural thrombosis and contribute to restenosis.

关 键 词：球囊血管成形术 PAI-1基因 基因表达 P-选择素 冠心病 再狭窄 

分 类 号：R540.5[医药卫生—心血管疾病]