凋亡相关蛋白TFAR19在TF-1细胞凋亡中出现细胞核转位  

Nuclear translocation of apoptosis related protein TFAR19 in TF-1 cells undergoing apoptosis

作　　者：陈英玉[1] 孙荣华[1] 韩文玲[1] 张颖妹[1] 宋泉声[1] 狄春辉[1] 马大龙[1] 

机构地区：[1]北京大学人类疾病基因研究中心,北京100083

出　　处：《北京大学学报（医学版）》2001年第2期97-100,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金 (3 9870 42 7)

摘　　要：目的 :探讨凋亡相关分子TFAR19在TF 1细胞凋亡过程中的表达及定位。方法 :以TFAR19的单克隆抗体为工具 ,采用荧光显微镜、共聚焦激光扫描显微镜、流式细胞仪等研究手段 ,观察细胞凋亡过程中TFAR19分子的表达水平和细胞内定位 ,分析其与细胞膜磷脂酰丝氨酸 (phosphatidylserine,PS)以及细胞核DNA片段化的关系。结果 :TFAR19蛋白在撤除GM CSF的TF 1细胞中表达水平显著增高 ,伴有明显的核转位现象 ,且早于PS外翻和细胞DNA片段化。结论 :TFAR19蛋白的核转位是细胞凋亡更早期发生的事件之一 ,这一新发现为进一步探讨TFAR19蛋白的生物学效应 。SUMMARY Objective: To study the expression and localization of apoptosis related protein TFAR19 in TF 1 cells undergoing apoptosis. Methods: Using monoclonal antibody against TFAR19, the expression level and cell localization of TFAR19 were examined by fluorescence microscope, confocal laser scan microscope(CLSM) and flow cytometry. Simultaneously, we also analyzed the relationship of TFAR19 protein with phosphatidylserine (PS) externalization and cell nuclear DNA fragmentation. Results: The level of TFAR19 proteins expressed in TF 1 cells treated with GM CSF withdrawal was significantly increased compared with normal TF 1 cells, then translocated rapidly from cytoplasm to the nucleus of cells. Appearance of TFAR19 in the nucleus of apoptotic cells preceded the detection of PS externalization and DNA fragmentation. Conclusion: Nuclear translocation of TFAR19 protein is one of the earliest events of cell apoptotic process. These data provided a new clue to further approach to the biological function of TFAR19 and study of cell apoptosis.

关 键 词：TFAR19 TF-1细胞 细胞凋亡 细胞核转位 

分 类 号：Q253[生物学—细胞生物学]