深低温保存的同种异体骨膜与胎骨联合移植的实验研究  

EXPERIMENTAL STUDY OF BONE REPAIR INDUCED BY CRYOPRESERVED ALLOGRAFT PERIOSTEUM AND FETAL BONE COMPOSITION IN BONE DEFECT

作　　者：许永涛[1] 顾洁夫[2] 尚平[1] 

机构地区：[1]荆州市中心医院骨科,湖北荆州434020 [2]湖北医科大学附属第二医院骨科

出　　处：《中国修复重建外科杂志》2001年第3期183-187,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：湖北省科委科研基金资助项目! (972 912 )

摘　　要：目的　探讨有生物活性的移植材料对骨缺损修复能力的影响。方法　实验采用兔桡骨缺损模型 ,经深低温保存的同种异体骨膜加胎兔骨移植为实验侧 ,胎兔骨移植为对照侧 ,分别在术后 2、4、8及 12周对实验侧和对照侧骨缺损模型进行大体观察、组织学检查、X线片及钙、磷含量检查。观察异体骨膜加胎骨复合体修复骨缺损的可行性及移植后骨膜、胎骨的动态变化。结果　实验侧术后 4、8及 12周钙、磷含量明显高于对照侧 (P<0 .0 5 ) ,随着时间的推移 ,钙、磷含量有升高的趋势。实验侧 8周可见髓腔再通 ,组织切片可见骨陷窝及板层骨形成。结论　胎骨是良好的移植材料。Objective To investigate the repairing effect of transplantation of allogeneic fetal bone in combination with a covering cryopreserved periosteal allograft to bone defect. Methods Twenty Long eared white male rabbits were chosen as experimental model of bilateral 12 mm combined bony and periosteal radial defect. Cryopreserved allograft periosteum with allogeneic fetal bone were implanted in the left defect as experimental side and fetal bone was simply transplanted in the right defect as control side. Bone repair process in the two groups were compared by macroscopy, microscopy, roentgenograms and the contents of calcium and phosphate in the defect area at 2、4、8 and 12 weeks after transplantation. Results There was significant statistic difference in the contents of calcium and phosphate between the experimental and control sides at 4、8 and 12 weeks after transplantation ( P <0.05). With time passing by, the contents of calcium and phosphate have the increasing trends. In the experimental group, lamella bone was seen and medullary canal recanalized at 8 weeks postoperatively. The histological section showed the bone lacuna and lamella bone were formed. Conclusion It suggests that allogeneic fetal bone in combination with a covering cryopreserved periosteal allograft can promote bone repair, and allogeneic fetal bone is excellent bone substitute.

关 键 词：深低温冻存 胎骨移植 骨缺损 修复 同种异体骨膜移植 

分 类 号：R687.34[医药卫生—骨科学]