肝细胞原代培养的技术改良及电镜观察  

The Improved Technology and Observed by Electron Microscopy on the Primary Culture of Hepatocyte

作　　者：曹献英[1] 闫玉华[1] 杨美娟[2] 张珠[1] 

机构地区：[1]武汉理工大学 [2]北京中医药大学

出　　处：《武汉工业大学学报》2001年第4期35-36,共2页

基　　金：国家自然科学基金资助(39770225).

摘　　要：为了评估无机纳米材料的疗效，设计了一种体外实验方法－等密度Ｐｅｒｃｏｌｌ离心法。该法是在Ｓｅｇｌｅｎ法分离肝细胞基础上改良的。实验表明，分离所得肝细胞形态、起微结构正常，具有较高的存活率和纶度。结果表明，这是一种有效、安全和可行的方法，便于操作，且不需要特殊的仪器设备。In order to estate the effect of inorganic nano material, an in vitro experiment method, a constant destiny Percoll centrifugal method is designed, which is based on the method of Seglen and improved by separating hepatocyte. The related ex- periment shows that the isolated hepatocyte by the method is normal in morphology and ultra structure, which have a high sur- vival rate and a high degree of purity. The result manifests that this method is very efficient, reliable and feasible, and more the method can be operated easily and need not special apparatus.

关 键 词：纳米材料 原代培养 SEM 肝细胞 恶性肿瘤 疗效 等密度Percoll离心法 

分 类 号：R318.08[医药卫生—生物医学工程]