长春花感染泡桐丛枝病原(MLO)后过氧化物同功酶的变化  

CHANGES IN PEROXIDASE ISOENZYMES IN PERIWINKLE INFECTED BY THE PAULOWNIA WITCHES' BROOM AGENT(MLO)

作　　者：田国忠[1] 金开璇[1] 汪跃[1] 

机构地区：[1]中国林业科学研究院林业研究所

出　　处：《林业科学研究》1990年第2期146-150,共5页Forest Research

基　　金：中国林业科学研究院科学基金

摘　　要：用聚丙烯酰胺凝胶电泳分析表明,长春花植株感染泡桐丛枝病原(MLO)后茎叶中过氧化物同功酶总谱带数呈减少的趋势,健株最多产生11条酶带,通常具有6条典型的、稳定的谱带,即Ⅰ_b、Ⅰ_c、Ⅰ_d、Ⅱ、Ⅲ_d和Ⅲ_e;而表现典型的Ⅲ级症状的病株缺少低Rf值区的Ⅲ_d和Ⅲ_e带。Ⅲ_d和Ⅲ_e酶带的有无、浓度及活性与病株外部症状的严重度成负相关。七叶期前的健株幼苗茎叶中也无Ⅲ_d和Ⅲ_e谱带;随着植株生理年龄的增长,其活性和浓度逐渐增大。土霉素处理病株可以提高Ⅲ_d和Ⅲ_e酶带的活性和浓度。Infection of periwinkle (yinca rosea) with paulownia witches' broom agent (mycoplasma like organism) by dodder resulted in regular changes in peroxidase isoenzymes. Peroxidases of leaves and stems in the healthy periwinkles were separated into 11 bands at most, generally 6 bands includingI b, Ic, Id, II, IIId and III. by polyacrylamide gel electrophoresis (PAGE). 4 band visible were shown in the infected plants with typical symptoms of yellow leaves, witches' broom and phyllody while band IIId and III. (with lower Rf value than other bands) could not be detected. It was confirmed that the concentration and width of band IIId and III. have close relation to the symptoms of periwinkle infected with PWB-MLO and ages of periwinkle. The healthy seedlings with less than 7 pairs of leaves were also lack of bandIIId and IIIe. The root immersion of diseased plants in oxytetracycline solution could make symptoms subside partially, and the result of PAGE of peroxid-ases showed that the concentration and activity of band IIId and III. in the treated plants were higher than the control (root immersion in distilled water) but still lower than the healthy plants (root immersion in oxytetracycline or distilled water). We deduced that the presence of band IIId and III. of peroxidase isoenzymes is a good indicator of the resistance of periwinkle against PWB-MLO.

关 键 词：泡桐 丛枝病 类菌原体 长春花 过氧化物同功酶 早期诊断 

分 类 号：S763.14[农业科学—森林保护学]