人尿红细胞生成素和血栓调节素的纯化研究  

Purification of human urine erythropoietin and thrombomodulin

作　　者：陶永辉[1] 金坚[2] 张荣军[1] 何扬[2] 张满达[1] 阮长耿[2] 

机构地区：[1]江苏省原子医学研究所核医学国家重点实验室,江苏无锡214063 [2]苏州医学院血栓与止血研究室,江苏苏州215007

出　　处：《中国生化药物杂志》2001年第2期57-60,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金! (39470 2 74)

摘　　要：目的从人尿中分离纯化红细胞生成素 (EPO)和血栓调节素 (TM )。方法用超滤浓缩、离子交换层析及亲和层析等方法从新鲜人尿中分别纯化EPO和TM。结果从 2 0 0 0kg新鲜人尿中分别纯化得到了 4.47mgEPO和 9.92mgTM ,得率分别为 31.9%和 2 5 .0 % ,分子量分别为 (38.6± 1.0 )kD和 (6 0± 1.4)kD ;等电点分别为3.6 0± 0 .0 2和 3 .70± 0 .0 2 ;TM富含Glu、Ala和Gly ,毛细管区带电泳分析高度均一 ,有关生化参数与文献报道基本一致。结论该工艺可获得纯度较高的EPO和TM。PurposeThe aim is to isolate and purify the erythropoietin(EPO)and thrombomodulin(TM) from human urine.MethodsPurifying and isolating human urine with means of filtration and concentration,ion exchange chromatography, affinity chromatography and so on.Results4.47 mg EPO and 9.92 mg TM were obtained from 2 000 kg human urine.Their molecular weights were(38.6±1.0)kD and (60±1.4)kD erespectively, the isoelectric point, 3.60±0.02 and 3.70±0.02, yield, 31.9% and 25.0%.The purified EPO and TM was shown to be highly homogeneous by capillary zone electrophoresis(CZE),abundant Glu,Ala and Gly include on TM. ConclusionThe high purity EPO and TM was obtained.

关 键 词：红细胞生成素 血栓调节素 尿 分离 亲和层析 

分 类 号：R446.122[医药卫生—诊断学]