高效液相色谱法测定人血浆中氟脲脱氧核苷的浓度  

Determination of floxuridine in plasma by HPLC

作　　者：黄品芳[1] 王长连[1] 刘亦伟[1] 陈希 

机构地区：[1]福建医科大学 [2]1999届药学专业实习生,

出　　处：《中国生化药物杂志》2001年第2期84-85,共2页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的建立高效液相色谱 (HPLC)法测定血浆样品中氟脲脱氧核苷 (FUDR)的方法。方法采用Nova pakC18色谱柱 ,0 .0 5mol/L磷酸二氢钠 甲醇 水 (0 .5∶7∶92 .5 )为流动相 ,检测波长 2 6 0nm ;用乙酸乙酯提取。结果在 0 .0 99～ 2 0 .0 μg/ml血药浓度范围内线性关系良好 ,r =0 .9994,三种浓度 (0 .2 3、1.6 7、2 0 .0 μg/ml)的回收率分别为 96 .4%、96 .5 %、97.8% (n =5 ) ;日内、日间RSD分别为 1.6 1%、1.98%、3 .17%和 3.5 6 %、1.90 %、2 .6 3 %。结论此法灵敏、准确 。Purpose The aim is to establish the HPLC method for the determination of Fluoro deoxyuridine in plasma.MethodsThe Chromatography conditions include: Chromatography column: Nova pak C 18 (3.9mm×150mm,4μm), mobile phase: 0.05mol/L sodium phosphate monobasic methanol water(0.5∶7∶92.5), UV detection at 260nm, FUDR was extracted with ethyl acetate. ResultsThe average recoveries were 96.4%,96.5%,97.8% for concentration 0.23、1.67、20.0μg/ml ( n =5).The corresponding reproducibility were RSD 1.61%, 1.98%, 3.17% respectwely for iner day and RSD 3.56%, 1.90%, 2.63% for the intra day( n =5). The FUDR concentration was linear with a correlation coefficient of 0.999 4 over the range of 0.099～20.0μg/ml. Conclusion The method was sensitive and accurate and suitable for pharmacokinetics and bioavailability study of FUDR.

关 键 词：氟脲脱氧核苷 高效液相色谱 血药浓度 

分 类 号：R979.1[医药卫生—药品]