丽格海棠叶片培养胚状体发生和植株再生  

Somatic Embryogenesis from Leaves of Begonia × Elatior

作　　者：达克东[1] 张松[1] 高东升[1] 米瑞芙[1] 周志文[1] 束怀瑞[1] 

机构地区：[1]山东农业大学园艺系,泰安271018

出　　处：《园艺学报》2001年第2期180-181,I001,共3页Acta Horticulturae Sinica

摘　　要：利用丽格海棠盆栽植株叶片培养 ,成功地诱导出胚状体并获得再生植株。具体步骤如下 :Ⅰ .在MS +BA 1mg/L +NAA 4mg/L + 2 ,4 D 1mg/L培养基上预诱导 10d ;Ⅱ .在MS +BA 1mg/L培养基上胚性细胞发生胚状体 ;Ⅲ .在MS +IBA 0 .5mg/L培养基上小植株生根。组织切片观察表明 。Somatic embryo formation was induced from leaves of Begonia × Elatior by a three step method:1.Embryonic cells were preliminary induced on MS+BA 1mg/L+NAA 4mg/L+2,4 D 1mg/L medium 10 days;2.Somatic embryo formation was observed on MS+BA 1mg/L medium;3.Roots were induced on MS+IBA 0.5mg/L medium.Histological study showed that the embryoid come from upper epidermal cell of the leaf.

关 键 词：秋海棠 组织培养 胚状体 

分 类 号：S682.19[农业科学—观赏园艺] S685.99[农业科学—园艺学]