缺血再灌注对前列腺内皮素基因表达的影响及药物干预  

Effect of ischemia/reperfusion on expression of endothelin in the rat ventral prostate and preventive measure

作　　者：尚平[1] 许宏[1] 高建平[1] 李芳秋[2] 

机构地区：[1]南京军区南京总医院泌尿外科,南京210002 [2]南京军区南京总医院检验科,南京210002

出　　处：《中国病理生理杂志》2001年第5期464-466,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :了解缺血再灌注对前列腺内皮素基因 (ET - 1mRNA)表达的影响及预防措施。方法 :用反复短暂钳夹腹主动脉的方法复制大鼠前列腺组织缺血再灌注模型 ,用RT -PCR方法检测ET - 1mRNA的表达。结果 :反复短暂缺血再灌注 6次共 90min后 1h和 3h ,前列腺组织中ET - 1mRNA的表达显著增加 (P <0 0 5 )。预防性应用地卓西平马来酸盐 (MK - 80 1)组 ,反复短暂缺血再灌注后 ,ET - 1mRNA的表达与对照无明显差异 (P >0 0 5 )。结论 :反复短暂缺血再灌注能使前列组织内皮素基因表达增加 ,这种反应可能在前列腺增生发生和发展病理过程中起一定的作用。预防性应用MK - 80 1可抑制缺血再灌注导致的内皮素基因表达增加。AIM: Effect of ischemia/reperfusion on expression of endothelin-1(ET-1) in the rat prostate and preventive measure were studied. METHODS: The abdominal aorta of rat was clipped briefly and repeatedly so as to treat the prostate with ischemia/reperfusion and expression of ET-1 mRNA in the ventral prostate was determined by RT-PCR.RESULTS: Expression of ET-1 mRNA in the ventral prostate was significantly increased at 1 h and 3 h after 90 min repeated ischemia/reperfusion ( P <0 05), and was not significantly changed after previous treatment of Dizocilpine maleate (MK-801) ( P >0 05). CONCLUSIONS: Expression of ET-1 in the prostate can be affected by repeated brief ischemia/reperfusion and it may play a role in the development of benign prostatic hyperplasia. Ischemia-reperfusion-induced ET-1 expression in the prostate of rats can be inhibited by prectreatment of MK-801.

关 键 词：前列腺增生 内皮缩血管肽类 动脉局部缺血 再灌注损伤 地卓西马来酸盐 

分 类 号：R697.32[医药卫生—泌尿科学]