猪后肢皮肤撕脱伤后TNFα和IL-1的检测意义  

Significance of detecting TNFα and IL-1 after skin avulsion of hind leg in pigs

作　　者：李向东[1] 鲁开化[1] 郭树忠[1] 高政慧[1] 

机构地区：[1]第四军医大学西京医院整形外科,西安710033

出　　处：《第三军医大学学报》2001年第5期562-564,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目! ( 394 70 70 5)

摘　　要：目的　探讨TNFα和IL 1在皮肤撕脱伤后早期继发血栓形成的作用。方法　在猪后肢形成 12cm× 4cm撕脱皮瓣 ,不同时间取皮肤并抽取静脉血 ,采用双抗夹心ELISA技术和3[H] TdR掺入法 ,检测血浆和皮肤中TNFα的含量和血清IL 1活性。结果　血浆和皮肤中TNFα含量明显升高 (P <0 .0 1) ,3d分别为 (415± 2 4)、(2 98± 18.5 )ng·L-1;血清中IL 1的活性 3d时也增高明显 (P<0 .0 5 ) ,为 (2 .5 9± 0 .85 )。结论　血浆、皮肤内TNFα和血清IL 1含量和活性变化在诱导。Objective To study the role of TNFα in the plasma and skin and IL 1 in the serum in the formation of secondary thrombosis after skin avulsion. Methods After avulsive flap at size of 12 cm×4 cm was inflicted on the hind legs of pigs, skin specimens and venous blood samples were taken at various time points. The contents of TNFα in plasma and skin were determined with radio immunoassay, and the activity of serum IL 1 with 3[H] TdR. Results The TNFα contents in the plasma and skin were increased significantly after avulsion( P <0.01),which were (415±24) ng/L and (298±18.5) ng/L respectively on the 3rd day after the injury. The activity of IL 1 in the serum was increased ( P <0.05) and was (2.59 ±0.85) ng/L on day 3. Conclusion The changes of TNFα contents and IL 1 activity in blood and skin play important roles in the inducetion and aggravation of secondary tissue necrosis and early thrombosis after skin avulsion.

关 键 词：肿瘤坏死因子-Α 白细胞介素-1 皮肤撕脱伤 猪 

分 类 号：R641[医药卫生—外科学] R622.1[医药卫生—临床医学]