溴化异丙托品抑制慢性支气管炎大鼠气道粘蛋白高分泌的观察  

Inhibition of airway mucin hypersecretion by ipratropium bromide in chronic bronchitis of rats

作　　者：刘维会 杨肇亨[1] 黄勇[1] 洪新[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所呼吸内科,重庆400042

出　　处：《第三军医大学学报》2001年第5期576-578,共3页Journal of Third Military Medical University

摘　　要：目的　探讨毒蕈碱乙酰胆碱能受体拮抗剂溴化异丙托品及阿托品对气道粘蛋白高分泌的作用。方法　采用一次性气管内注入脂多糖复制大鼠慢支炎模型 ,观察 3周后的病理学改变、支气管肺泡灌洗液和气管环培养液中粘蛋白量的变化 ,以及雾化吸入溴化异丙托品及阿托品的影响。结果　注入脂多糖后的病理学表现符合慢支炎的改变。溴化异丙托品和阿托品都显著减少气管上皮杯状细胞数目及粘蛋白的分泌。但溴化异丙托品抑制粘蛋白分泌的作用显著强于阿托品。结论　毒蕈碱乙酰胆碱能受体途径在慢支炎大鼠气道粘蛋白的高分泌中起重要作用 ,溴化异丙托品明显抑制粘蛋白高分泌 ,其作用强于阿托品 。Objective To investigate the effect of ipratropium bromide and atropine on airway mucin hypersecretion in the chronic bronchitis model of rats. Methods The model was established by intratracheal instillation of small dose of lipopolysaccaride (200 μg) ipratropium bromide and atropine were administrated 3-21 d after the model was established. Pathological changes, mucin in bronchoalveolar lavage fluid (BALF) and tracheal ring culture medium were examined before and after the adnsinistration of ipratropium bromide and atropine. Results Characteristic pathological manifestions of chronic brochitis were found after instillation of LPS. Significant decrease in the number of tracheal epithelia goblet cells, secretion of mucin were observed in ipratropium bromide and atropine treated rats. The effect of inhibiting the secretion of mucin of ipratropium bromide was higher than atropine. Conclusion It suggests that muscarinic acetylcholine receptor plays an important role in airway mucin hypersecretion in chronic bronchitis model of rat. Ipratropium bromide exhibit a stronger inhibition effect on mucin hypersecretion than atropine, moreover no inhibition effect on mucociliary clearance which was observed in atropine.

关 键 词：慢性支气管炎 粘蛋白 溴化异丙托品 阿托品 毒蕈碱乙酰碱能受体 大鼠 

分 类 号：R562.21[医药卫生—呼吸系统]