^(32)P液体充盈球囊照射预防猪冠状动脉球囊损伤后细胞增殖和内膜增生  

IRRADIATION WITH ^(32)P LIQUID FILLED BALLOON TO PREVENT CELL PROLIFERATION AND NEOINTIMAL FORMATION AFTER CORONARY ANGIOPLAST IN SWINES

作　　者：文应峰[1] 盖鲁粤[1] 张端珍[2] 曾永寿 

机构地区：[1]解放军总医院 [2]解放军第208医院 [3]云南楚雄州人民医院

出　　处：《解放军医学杂志》2001年第5期339-341,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：选择小型猪 15头 ,对左前降支近中段行过度球囊扩张术 ,术后 7头行3 2 P液体充盈灌注球囊照射 ,剂量为 2 4Gy ,另 8头为对照。术后 35天目标血管行常规苏木素和伊红等染色 ;并免疫组织化学染色分析增殖细胞核抗原 (PCNA)和α smoothmuscleactin(α SMA)。通过计算机图像分析系统测定血管形态、细胞增殖百分比等。结果显示 :放疗组管腔面积较对照组明显增大 ,新生内膜面积、血管狭窄程度明显减少 (P均 <0 0 1) ;放疗组较对照组内膜、中膜、外膜PCNA阳性细胞百分比明显减少 (P均 <0 0 1)。该方法操作简单 ,成功率高 ,结果表明放射性液体充盈灌注球囊行冠脉内照射预防再狭窄是有效、安全和可行的。miniature swines were randomly divided into irradiation group ( n =7) and control group ( n =8). Immediately after balloon overstretch injury to LAD, radioactive liquid perfused balloon irradiation was performed at the target segment; radioactive dose was 24Gy. 35 days after the operation, the target segments were harvested to perform histologic and morphologic study (HE, MS, VVG) and immunohistochemical study (PCNA, α smooth muscle actin). Results showed that the lumen area was significantly larger, the neointima area and vascular stenosis level were smaller, and less PCNA positive cells were present in the vascular wall in the irradiation group than in the control group ( P all<0.01). The results of the study suggested 32 P liquid filled balloon was easy to operate, the procedure was safe, effective and feasible in preventing restenosis.

关 键 词：细胞增殖 内膜增生 冠状动脉球囊损伤 放射性液体球囊系统 

分 类 号：R654.2[医药卫生—外科学]