石蜡包埋组织切片原位核酸杂交研究  

Study of hybridization in situ in paraffin-embedded myocardial specimens

作　　者：任立群[1] 赵志涛[1] 孙波[1] 李广生[1] 朴松旭[2] 

机构地区：[1]吉林大学生物工程研究所病理研究室,吉林长春130021 [2]延边大学附属医院中心实验室

出　　处：《白求恩医科大学学报》2001年第3期235-237,共3页Journal of Norman Bethune University of Medical Science

基　　金：国家自然科学基金资助项目! (39870 6 6 8

摘　　要：目的 :探讨石蜡包埋组织切片的原位核酸杂交方法 ,以检测克山病 ( KSD)尸检心肌中的RNA。方法 :应用生物素标记的人工合成寡核苷酸探针 ,对 72例心肌石蜡包埋组织切片进行原位核酸杂交。结果 :各型克山病心肌石蜡组织切片的阳性杂交信号为 80 %～ 87.9% ,而正常对照仅为 14.3%。结论 :应用人工合成寡核苷酸之原位核酸杂交技术 ,对长期保存的石蜡组织进行回顾性研究是可行的。Objective: To explore the method of hybridization in situ in paraffin embedded myocardial specimens in order to examine RNA of Coxsackie virus of myocardial tissue from patients with Keshan disease(KSD). Methods: Hybridization in situ was performed by the use of the oligonucleotide probe by artificial synthesis labelled with biotin.Results:Positive hybrid signal was found in paraffin embedded myocardial specimens. The positive rates in acute,subacute and chronic KSD were 83%(10/12),87.9%(29/33) and 80%(16/20) respectively, but only 14.3%(1/7) in control.Conclusion: It is feasible to perform the reviewable study on paraffin embedded tissue kept for long term using hybridization in situ technology. [

关 键 词：原位核酸杂交 寡核苷酸探针 石蜡包埋组织 肠道病毒 克山病 KSD RNA 

分 类 号：R542.3[医药卫生—心血管疾病] R361.2[医药卫生—内科学]