燕麦对冠锈病水平抗性的QTL定位  

Identification of QTLs for horizontal resistance to crown rust in oat

作　　者：陈钢[1] HowardWRines DeonDStuthman KurtJLeonard 丁守仁[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029 [2]Dept.ofAgronomyandPlantGenetics [3]CerealDiseaseLaboratory

出　　处：《浙江大学学报（农业与生命科学版）》2001年第2期151-155,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：美国 Quaker Oat公司资助项目

摘　　要：为了确定与燕麦冠锈病水平抗性基因相关的分子标记 ,建立了具水平抗病性的品系MN84 180 1- 1和感病品种 Noble- 2杂交的 F8群体 .该群体由 158个重组自交系组成 .用 112个 RFLP标记构建了其连锁图 ,该图由 17个连锁群 (A- Q)组成 ,另有 2 3个位点未连锁 ,大约覆盖了燕麦基因组的 1498.2 c M.重组自交系群体的抗病性鉴定分别在温室和田间进行 .用病斑面积占总叶面积的百分率(PPA)和病害发展曲线下的面积 (AUDPC)作为抗病指标 ,在田间试验中分别探测到 3个燕麦冠锈病水平抗性的 QTL,依次命名为 PRQ1,PRQ2和 PRQ3.这 3个 QTL分别位于连锁群 C,P和 N上 ,总共可解释 2 7%的表现型变量 .在温室试验中只探测到 PRQ1和 PRQ2两个 QTL,当用 AUDPC和 PPA分别作为抗病指标时 ,这两个 QTL可分别解释 32 %和 2 6In order to identify molecular markers associated with genes for horizontal resistance to crown rust in oat, a population consisting of 158 F8 recombinant inbred lines from a cross of ‘MN841801 1 × Noble 2’ was mapped with 112 RFLP loci. Alinkage map was constructed into 17 linkage groups and 23 unlinked loci. Disease resistance was evaluated on adult plants in greenhouse and field. Three horizontal resistance QTLs, termed PRQ1, PRQ2 and PRQ3, located on linkage groups C, P and N, respectively, were identified for area under the disease progress curve (AUDPC)and for percentage of pustule area versus total leaf area (PPA) in field and together explained 27% of the phenotypic variance. Two of these QTLs (PRQ1 and PRQ2) were identified in the greenhouse tests and explained 32% and 26% of the phenotypic variance when assessed as AUDPC and PPA.

关 键 词：燕麦 冠锈病 数量性状位点作图 水平抗病性 QTL定位 抗病指标 PPA AUDPC 

分 类 号：S512.6[农业科学—作物学] Q348[生物学—遗传学]