大鼠小肠循环液中替硝唑的差示分光光度测定法  

Determination of tinidazole in rat small intestine circulation solution using differential spectrophotometry

作　　者：李晶[1] 吴宏斌[1] 陈义 史春胜 

机构地区：[1]佳木斯大学化学与药学院 [2]齐齐哈尔市医药公司 [3]佳木斯中药厂

出　　处：《黑龙江医药科学》2000年第3期18-19,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的：建立大鼠小肠液循环液中的替硝唑的测定方法。方法：采用差示分光光度法．测定波长为318nm和291nm，溶剂的pH分别为pH2和pH5。结果：标准曲线在4．8μg／ml－19．2μg／ml范围内呈良好的线性关系．高中低3个浓度的日内RSD小于1．65％，日间RSD小于2．28％，回收率为98．6％－101．7％．结论：该法可准确测定大鼠小肠循环液中的替硝唑．Objective: To establish the determination of tinidazole in rat small intestine circulation solution. Methods:Differential spectrophotometry was used with wavelength of 291nm or 318nm with. The solvent pH as 2.0 or 5.0. Results: The calibration curve was linear between 4.8μg/ml to 19.2μg/ml. The relative standard deviations within a day and day to day were less than 1.65% and 2.28% at the three different concentraions. The recovery was 98.6% to 101.7%. Conclusion: Tinidazole in rat small intestine cboulation solution can be determined accurately by this method.

关 键 词：替硝唑 差示分光光度法 酚红 大鼠小肠循环液 

分 类 号：R978.61[医药卫生—药品]