谷氨酸致伤后神经元胞内游离钙的动态变化  

Disturbance of calcium homeostasis induced by glutamate in cultured neurons of rat cerebral cortex

作　　者：朱晓峰[1] 夏一鲁[2] 张拥波[2] 谭丽萍 

机构地区：[1]佳木斯大学医学院神经疾病研究所 [2]重庆医科大学神经病学研究所

出　　处：《黑龙江医药科学》1999年第5期1-1,共1页Heilongjiang Medicine and Pharmacy

摘　　要：目的：探讨谷氨酸致伤后大脑皮层神经完胞内游离钙的动态变化特征．方法：大鼠大脑皮层神经元原代分离培养方法和光电联合检测技术，培养8天的神经元用于实验．结果：谷氨酸致伤2h内，神经元胞内游离钙降低，60min、90min组比对照组明显降代，2h后胞内游离钙升高，以210min、480min组升高显著．结论：Ca2+稳态破坏参与谷氨酸致伤过程，不仅是Ca2+超载，早期Ca2+降低值得重视．Objective: To observe the effect of glutamate on the calcium homeostasis of cultured rat cortical neurons. Method:Single neuron culture technology and light-electron measurement system. The cultured neurons in 8 day were uscd the experiment.Result: The Ca2+ level was decrease in 2h after glutamate exposure The level was lower than that of control in 60min and 90min but higher in 210min and 480min. Conclusion:disturbance of calcium homeostasis is contributed to the neuron injure induced by glutarnate. The Ca2+ decrease in the early period has value in neuron injure. The cytosolic free calcium Ca2+ change processes were observed at the time points of 50, 90, 120, 150, 210 and 480 min after exposure to 300μmol/L glutamate for 5 min.

关 键 词：谷氨酸 神经元 游离钙 药理 

分 类 号：R977.6[医药卫生—药品]