荔蝽中乙酰胆碱酯酶活性与自然抗药性的关系  

RELATIONSHIP BETWEEN THE ACTIVITY OF ACETYLCHOLINESTERASE AND THE TOLERANCE TO INSECTICIDE IN THE LYCHEE STINK BUG

作　　者：陈文奎[1] 赵善欢[1] 

机构地区：[1]华南农业大学昆虫毒理研究室

出　　处：《华南农业大学学报》1991年第4期18-24,共7页Journal of South China Agricultural University

基　　金：国家教委高等院校博士点基金

摘　　要：本文研究了荔蝽头部乙酰胆碱酯酶(AChE)活性的季节性变化与自然抗药性的关系,以及敌百虫对AChE的影响,同时运用聚丙烯酰胺凝胶电泳的方法,探讨了荔蝽酯酶同功酶的变动情况。结果表明:荔蝽正常成虫头部AChE活性在新羽化成虫中最高,比活力达3.5μmol min^(-1)mg^(-1)蛋白质,越冬期最低,仅1.17μmol min^(-1)mg^(-1)蛋白质,到生殖期又上升为2.67μmol min^(-1)mg^(-1)蛋白质。经敌百虫处理后,在新羽化成虫期和生殖期AChE活性有明显被抑制现象,其比活力分别为2.21μmol min^(-1)mg^(-1)蛋白质和2.04μmol min^(-1)mg^(-1)蛋白质,而在越冬期AChE活性几乎不受敌百虫影响,为1.24μmol min^(-1)mg^(-1)蛋白质,同功酶谱也显示AChE在不同季节中无明显变化。The acetylcholinesterase (AChE) activity of the Lychee stink bug, Tessaratoma papillosa Drury was determined during different seasons. The activity of AChE was 3. 5 Mmol/min/mg potein during the newly emerged period, 1. 17 μmol/min/mg protein during the overwintering period and 2. 67 μmol/min/mg protein during the reproductive period , respectively. Results of detailed laboratory studies showed that the tolerance to trichlorfon is caused by the insensitivity of the AChE to the insecticide. Dynamic changes of AChE and esterase isozymes were studied by means of polyacrylamide gel electrophoresis. Scanning of electrophoresis zymograms showed that no changes took place in the AChE zymograms.

关 键 词：荔蝽 乙酰胆碱酯酶 抗药性 荔枝 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]