普通野生稻胚性细胞悬浮系的建立及原生质体再生植株  

ESTABLISHMENT OF EMBRYOGENIC CELL SUSPENSION CULTURES AND PLANT REGENERATION FROM PROTOPLASTS OF WILD RICE (ORYZA RUFIPOGON)

作　　者：张伟[1] 简玉瑜[1] 

机构地区：[1]华南农业大学实验中心遗传工程研究室

出　　处：《华南农业大学学报》1991年第4期13-17,共5页Journal of South China Agricultural University

摘　　要：广西普通野生稻(Oryza rufipogon)2个品种的成熟种子经54±1℃温度处理5d打破休眠后,诱导产生出愈伤组织。挑选胚性愈伤组织置于液体培养基中振荡培养。经6个月继代培养,建立起胚性细胞悬浮系。其中1个品种(No.40)的悬浮细胞经酶解游离获得大量原生质体,用琼脂糖包埋培养,2周内分裂频率达21.4％。形成的小愈伤组织经增殖后在分化培养基上再生出绿色小植株。Calli were derived from the seeds of Guangxi wild rice (Oryza rufipogon) No. 40 and 68-w172 their dormancy being broken by heat treatment(54 ± 1℃,for 5 days). Suspension cell cultures were established by selecting embtyogenic calli and culturing them in liquid medium on shaker for six months. High yield protoplasts were isolated from the suspension - culture cells of the wild rie No. 40 and imbedded in agrose to grow. After two weeks, the division frequency rose to 21. 4%. Small callus clumps devived from protoplasts were transferred onto regeneration medium and from then green plantlets were obtained.

关 键 词：野生稻 原生质体 植株再生 

分 类 号：S511.903.5[农业科学—作物学]