盐酸哌唑嗪点眼房水葡萄膜巩膜途径的形态观察  

Morphological study of uveoscleral pathway of aqueous humor drainage following topical prazosin in rabbit eye

作　　者：孙乃学[1] 喻应贵[1] 王建明[1] 赵世平[1] 

机构地区：[1]西安医科大学第二附属医院眼科

出　　处：《眼科研究》1998年第1期10-12,共3页Chinese Ophthalmic Research

基　　金：国家自然科学基金

摘　　要：目的观察兔眼房水葡萄膜巩膜途径的形态，探讨选择性α１受体阻断剂盐酸哌唑嗪（ｐｒａｚｏｓｉｎ，ＰＺ）点眼对该途径的形态学影响。方法前房注入微量示踪剂异硫氰酸荧光素牛血清白蛋白（ｆｌｕｏｒｅｓｃｅｉｎｉｓｏｔｈｉｏｃｙａｎａｔｅ－ｂｏｖｉｎｅｓｅｒｕｍａｌｂｕｍｉｎ，ＦＩＴＣ－ＢＳＡ）于ＰＺ点眼后２，４，６，８，１０，１２ｈ各处死家兔２只，摘除双侧眼球作冰冻切片，荧光显微镜下观察并确定睫状体、脉络膜上腔、前后巩膜和脉络膜的荧光强度等级。光镜下观察其组织结构变化。结果ＰＺ点眼后眼压出现有统计学意义的下降。ＰＺ点眼后葡萄膜巩膜途径各部位的荧光强度均比对照组显著增强，光镜观察仅见ＰＺ组睫状肌细胞间隙扩大。结论ＰＺ点眼可使眼压下降，点眼后促进房水从葡萄膜巩膜途径排出，房水由睫状肌细胞间隙到脉络膜上腔后，主要经前巩膜排出，后巩膜及脉络膜排出较少。ObjectiveTo　observe　the　morphology　of　the　uveoscleral　pathway　of　aqueous　humor　drainage　and　to　discuss　the　influence　on　this　pathway　of　topical　prazosin　(PZ).MethodsWith　microamounts　of　fluorescein　isothiocyanatebovine　serum　albumin　(FITCBSA)　infused　into　anterior　chamber　of　rabbit　eye　as　the　tracer,two　rabbits　in　each　group　were　killed　at　2,4,6,8,10,and　12　h　after　PZ　instillation.Their　eyeballs　were　removed　and　frozen　sections　were　performed.Degrees　of　fluorescent　intensity　of　ciliary　body,suprachoroidal　space,anterior　and　posterior　sclera,and　choroid　were　observed　under　fluorescence　microscopy.Histological　structures　of　these　sites　were　also　observed　under　light　microscopy.ResultsIOPs　showed　a　statistical　decrease　following　topical　PZ.Compared　with　control　group,fluorescent　intensities　of　all　uveoscleral　pathway　sites　were　significantly　stronger　after　PZ　instillation.With　light　microscopy　we　only　found　that　ciliary　intramuscular　spaces　were　increased　in　PZ　group.ConclusionPZ　can　decrease　IOP　following　topical　application.The　morphological　changes　have　shown　that　topical　PZ　promotes　uveoscleral　outflow.Aqueous　humor　flows　into　the　suprachoroidal　space　through　the　interstitial　spaces　of　the　ciliary　muscle　and　out　through　anterior　sclera　predominantly.Smaller　amounts　of　aqueous　humor　leave　the　eye　through　posterior　sclera　and　choroid.

关 键 词：盐酸哌唑嗪 葡萄膜巩膜途径 眼压 PZ点眼 降眼压作用 兔 

分 类 号：R988.1[医药卫生—药品]