磷脂酰肌醇特异性磷脂酶C活力的非同位素酶解测定法  

A NONISOTOPIC ENZYMOLYSIS ASSAY OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C

作　　者：张泽林[1] 刘武[2] 

机构地区：[1]华西医科大学药学院,成都610041 [2]华西医科大药学院

出　　处：《华西药学杂志》1991年第1期41-43,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金.

摘　　要：作者以磷脂酰肌醇为底物,经特异性磷脂酶C水解后,分离产物二酰基甘油及肌醇一磷酸,再用碱性磷酸酶水解肌醇一磷酸的磷酸单酯键,用高灵敏度的磷测定法测定磷含量,建立了磷脂酰肌醇特异性磷脂酶C活力的非同位素酶解测定法。该法简便易行,C·V=5.8%。Phosphotidylinositol(PI)was used as the substrate,after hydrolyzed by phosphoinositide-specific phospholipase C(PI-PLC),the products,diacylglycerol (DG)and inositol monophosphate(IP)were separated.The phosphate was hydrolyzed from IP by using alkaline phosphatase.Then,determine the enzyme activity by assay the content of phosphate.No isotope was used in this method.C.V=5.8%.

关 键 词：磷脂酶C 活力测定 

分 类 号：R927.1[医药卫生—药学]