Localization of Neuropeptidelike Substances During Embryonic Development in Amphibian  

神经肽样物质在两栖类胚胎发育过程中的定位研究(英文)

作　　者：张小云[1,2] 罗振国[1,2] 曾弥白[1,2] 

机构地区：[1]深圳大学生命科学研究室 [2]中国科学院上海细胞学研究所

出　　处：《Developmental and Reproductive Biology》1997年第2期1-12,共12页发育与生殖生物学报（英文版）

摘　　要：The localization of four neuropeptidelike substances during embryonic development in amphibian was studied by using immuno cytochemical technique. The cells with positive reaction appeared firstly in the endoderm cells during early tailbud stage, and then were detected in connective tissue at the outer portion of gastrointestinal tract during tadpole stage. In the nervous system, the cells with positive reaction were observed in cranial ganglion and glial cells at the outer margin of the brain and in the inner wall of ventricles. They were also frequently observed in the epidermis during late tailbud stage. The relationship between the appearance of neuropeptides in timespatial sequences and the development of nervous system, the neural crest origin of the cells with positive reaction, and the role of epidermal conductivity in neuropeptidelike cells in epiderms were discussed.本工作以东方蝾螈为实验材料，选用时期为：卵裂期，神经胚早、中、晚期，尾芽早、中、晚期，及蝌蚪期等８个时期。各期胚胎经过固定、ＯＣＴ包埋、切片后，分别做抗胰岛素、抗胆囊收缩素、抗生长素和抗β内啡肽等免疫反应，ＡＢＣ法染色处理并观察。结果发现：一、在胚胎发育的一定时期，阳性细胞出现在胚胎的一定部位。最早于尾芽早期出现在内胚层，此时，个别细胞呈阳性反应。尾芽中期阳性细胞明显增多（Ｆｉｇ．１）。到蝌蚪期，在成形的肠道外缘可以看到阳性细胞（Ｆｉｇｓ．２＆３），躯干部内胚层的外缘也有呈阳性反应的细胞（Ｆｉｇ．４）。可以认为：胚胎发育过程中，神经肽物质出现的时空程序与神经系统发育不相平行。从卵裂期到神经胚期，神经管从出现到闭合的管内外，都找不到阳性物质的存在。直到尾芽早期才发现神经肽样物质，而且分布在内胚层。到尾芽中期，可在表皮中发现。而神经系统则到了尾芽晚期，也就是神经管闭合后８６小时，在眼杯后才出现阳性细胞。到蝌蚪期，神经肽样物质仅存在于周边神经系统。二、在神经系统，阳性细胞最早出现在尾芽晚期，在眼杯后方可以观察到成群的阳性细胞。该位置很可能是脑神经节（Ｆｉｇ．５）。蝌蚪期，神经系统从前端往后都可观察到阳性细胞，它?

关 键 词：neuropeptidelike substance embryonic development neural crest epidermal conductivity AMPHIBIAN immunocytochemistry. 

分 类 号：Q132.4[生物学—普通生物学] Q959.5