In　situ　Localization　and　Isolation　of　Actin　Filaments　from　Pollen　Tubes　of　Amaryllis　vittata　Ait  

作　　者：蔡雪 董云洲 

机构地区：[1]CollegeofLifeSciences,PekingUniversity,Beijin100871 [2]BiotechnologyResearchCenter,ChineseAcademyofAgric

出　　处：《Developmental and Reproductive Biology》1994年第2期64-69,T001,T002,共8页发育与生殖生物学报（英文版）

摘　　要：Actin filaments (AFs) in un-fixed pollen tubes of Amaryllis vittata Ait were visualized after TRITC-phalloidin staining with DMSO as a permeabilising agent. Typically, strands or hundles of microfilaments (Mfs) were distributed in the extreme tip as well as pollen tubes in a form of network.Fluorescent granules or circles of various sizes were frequently found that continued with the filamentous structures. In addition, a more brightly stained structure, possibly Mf organizing center, was observed. Treatment of pollen tubes with cytochalasin D(CD)for increasing time intervals (5-40 minutes) caused gradual reduction of strands until flurescent granules filled up the pollen tubes. Mcanwhile, cytoplasmie streaming was inhibited completely. Though closely associated with vegetative nuclei (VN) and generative cells (GC), AFs were not found in the cytoplasm of GC.Mg++concentration greatly affected the isolated Mfs.ＩｎｓｉｔｕＬｏｃａｌｉｚａｔｉｏｎａｎｄＩｓｏｌａｔｉｏｎｏｆＡｃｔｉｎＦｉｌａｍｅｎｔｓｆｒｏｍＰｏｌｌｅｎＴｕｂｅｓｏｆＡｍａｒｙｌｌｉｓｖｉｔｔａｔａＡｉｔＣＡｌＸｕｅ（蔡雪）；ＤＯＮＧＹｕｎ－ｚｈｏｕ（董云洲）（ＣｏｌｌｅｇｅｏｆＬｉｆｅＳ...

关 键 词：Actin filament Pollen tube TRITC-phalloidin Cytochalasin D ISOLATION Mg￣(++) Amaryllis vittata Ait. 

分 类 号：Q945.64[生物学—植物学] Q944.42