热休克反应中小鼠心脏HSP70表达的免疫组织化学研究  

The Immunohistochemical Study of HSP70 Expression of the Mouse Heart in Heat Shock Response

作　　者：郭玫[1] 张顺利[1] 张文学[1] 魏纪东[1] 张新胜[1] 薛德明[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《动物学杂志》2001年第2期15-18,共4页Chinese Journal of Zoology

基　　金：中法先进技术合作项目!(B96 11);河南省教委自然科学基金项目!(No .97180 0 14)

摘　　要：用免疫组织化学方法研究了小鼠心脏在不同温度 ( 40、4 2、4 4、4 6℃ )热休克处理后 ,各恢复期 ( 2、4、8、1 2、2 4小时 )HSP70的表达。结果表明 ,( 1 ) 4 4、4 6℃热处理能诱导心肌细胞合成HSP70 ,以 4 6℃为多 (P<0 0 1 ) ,且于恢复期 4～ 8小时为合成高峰 (P <0 0 1 ) ;( 2 )阳性免疫反应定位于心肌细胞质中 ,核呈阴性反应。提示了心脏有较强的耐热能力。The immunohistochemical method was used to analyze the expression of HSP70 in the heart of the mice which were treated at different temperature (40,42,44,46℃) for 30 minutes and had different recovery time (2,4,8,12,24 h). The results show that: (1) Cardiac muscle cells could synthesize HSP70 at 44℃ and 46℃, HSP70 was increased in 46℃ group ( P <0 01). There was peak value of HSP70 after 4～8 h recovery( P <0 01). (2) Positive immunoreactivity was localized in the cytoplasm while nucleus was negative. It suggests that the heart have strong thermotolerance in the heat shock.

关 键 词：小白鼠 心脏 免疫组织化学 热休克蛋白70 

分 类 号：Q256[生物学—细胞生物学] Q494