Mab-ELISA对IBV毒株定性检测方法的建立与应用  

Mab-ELISA for Identification of Avian Infectious Bronchitis Virus and Its Application

作　　者：王林川[1] 黄爱芳[1] 游洪[1] 

机构地区：[1]华南农业大学动物医学系,广东广州510642

出　　处：《华南农业大学学报》2001年第1期74-77,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金!重大项目 (398932 90 - 2B)

摘　　要：针对禽传染性支气管炎病毒 (IBV)血清型众多、临床检测不易的难题 ,用我国自己研制的 2株IBV单克隆抗体 ,通过多种方法的比较与鉴定 ,建立起适用于定性检测IBV的包被鼠源IBV单抗的Mab ELISA方法 ,其操作程序是 :包被鼠源IBV单抗 -被检病毒 -鸡抗IBV高免血清 -兔抗鸡IgG (AGP效价 1∶32 ) -羊抗兔IgG -HPR ;用本方法对国内 46个IBV分离株及NDV、AIV H9N2、PPMV I等的检测 。This study established an identification procedure for the avian infectious bronchitis virus, MabELISA, with two strains of IBV monoclonal antibody made by an institution in China. The procedure was: coating IBV Mab (murine)test sample (virus)IBV positive serum from chicken-antichicken IgG from rabbit (AGP titres 1∶32)-sheep antirabbit IgGHPR. The check using 46 IBV isolates from China with NDV, AIVH9N2 and PPMVI suggested that this IBV MabELISA method had relatively good specificity and sensitivity.

关 键 词：禽 传染性支气管炎病毒 单克隆抗体 酶联免疫吸附测定法 定性检测 传染性支气管炎 

分 类 号：S852.659.6[农业科学—基础兽医学]