高效液相色谱测定小麦抗锈与非抗锈品系水杨酸及H_2O_2含量的变化  

HPLC detect SA and H_2O_2 content in wheat of incompatible and compatible reaction to Puccimia striiformis f. sp. Tritici

作　　者：陈勇胜[1] 

机构地区：[1]甘肃农业大学成人教育学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2001年第1期75-78,共4页Journal of Gansu Agricultural University

摘　　要：高效液相色谱（HPLC）测定结果表明，接种后的抗锈品系95672的水杨酸（SA）与过氧化氢（H2O2）含量均有所升高；不抗锈甘麦8号品种接种锈菌后SA含量也有升高，分别为148及32μg/gFW，但H2O2含量并无明显变化，推测SA在为过氧化氢酶（CAT）的过氧化活性提供一个电子的过程中SA的量必须达到一定的阀值，并且与CAT的时序调节相配合，根据SA结合态与游离态含量的变化推测SA信号传导途径的上游应有其它机制在起作用，并且该抗锈品系具有SA信号传导途径。：The result of HPLC shows that the SA and H2O2 content are increased in the plant' body of a new variety of wheat —No. 95672. Although the SA content arise in 'Gan Mai No.8'，H2O2 content didn't change. Infect the progress of SA give e- to CAT need the SA content attain a key content and change SA content is at the right moment of CAT activity. Depend on change free SA content and combine SA content the conclusion are deduced that SA signaling pathway have some things on the upstream, 95672 have SA signal pathway.

关 键 词：高效液相色谱 水杨酸 过氧化氢 小麦 含量测定 锈病 抗病机制 

分 类 号：S512.101[农业科学—作物学] S435.121.4