PSⅡ的荧光光谱特性  

FLUORESCENCE SPECTRUM CHARACTERISTIC OF PSⅡ

作　　者：贺俊芳[1] 王水才[1] 张舒[1] 贺锋涛[1] 任兆玉[1] 陈耀东[2] 单际修[2] 匡廷云[2] 

机构地区：[1]中国科学院西安光学精密机械研究所瞬态室,西安710068 [2]中国科学院植物研究所,北京100093

出　　处：《光子学报》2001年第3期257-261,共5页Acta Photonica Sinica

基　　金：国家重点基础研究专项!( G1 9980 1 0 1 0 0 );所长基金资助项目

摘　　要：采用激励光源为 82 MHz、51 4.5nm的皮秒荧光光谱装置对 PS 颗粒、内周天线 CP43、CP47三种样品进行研究 ,通过探测三种样品的荧光总光谱强度随激光功率的变化 ,测得 PS 颗粒样品在激光功率为 1 2 0 m W时 ,荧光强度趋于饱和 ;CP43在激光功率为 73m W时 ,荧光趋于饱和 ,但当激光功率为 82 m W时 ,荧光强度有下降趋势 ;而在激光功率为 2 0～ 96m W的范围内 ,CP47的荧光强度与激光功率几乎是线性关系 .依据它们的荧光量子产额与激光功率的关系 ,认为 CP47中存在较强的激子效应 .几种样品的荧光光谱范围分别为 70 0～780 nm(PS 颗粒 ) ;640～ 780 nm(CP43) ;630～ 775nm(CP47) .CP43和 CP47的最大荧光峰分别为 680 nm和 690 nm,荧光寿命分别为 3.543ns和 3.2 2 2 ns.在 51 4.5nm激光激发下 ,CP43和 CP47中最先受激发的是 β- Car分子 ,发射荧光的是 Chla分子 ,理论计算认为在CP43和 CP47中 Chla分子发射荧光的效率分别为 38.3%和 40 .6% .PSⅡ particles,internal antenna CP43 and CP47 were studied by using picosecond fluorescence spectroscopy with 82MHz,514 5nm exciting light The change of fluorescence intensity with laser power shows that fluorescence intensity of PSⅡ particles becomes saturation when laser power is 120mW CP43′s fluorescence becomes saturation when laser power is 73mW but has fall trend as laser power reach 82mW,CP47′s fluorescence is almost linear with laser power in 20～96mW The relations between fluorescence quantum yield and laser power indicate CP47 exists strong exciton effect Fluorescence spectrum scales of several samples are 700～780nm for PSⅡ particles,640～780nm for CP43,630～775nm for CP47 The most peaks of CP43 and CP47 are～680nm and ～690nm,and the fluorescence lifetimes are 3 543ns and 3 222ns respectively Excited by 514 5nm laser the primary excited molecular of CP43 and CP47 are β Car,and emission fluorescence molecular is Chla Academic calculation indicted Chla emission efficiency of CP43 and CP47 are 38.3% and 40 6% separately

关 键 词：PSⅡ CP43 CP47 能量传递 荧光 激子效应 光系统Ⅱ 光合作用 

分 类 号：Q945.11[生物学—植物学] Q631