氟化物及氟化物加钙对卵巢切除大鼠血清中LPO变化的影响  

Effect of fluoride and fluoride plus calcium on serum lipid peroxide in ovariectomized rats

作　　者：赵雅君[1] 闫景龙[2] 娄延平[1] 姜晓姝[1] 富德[2] 王志成[2] 

机构地区：[1]哈尔滨医科大学病理教研室,哈尔滨150086 [2]哈尔滨医科大学附属二院骨外科

出　　处：《中国骨质疏松杂志》2001年第2期140-142,共3页Chinese Journal of Osteoporosis

摘　　要：目的　确定氟化物及钙对卵巢切除大鼠血清脂质过氧化物的影响。方法　 6 4只雌性Wistar大鼠 ,随机分为卵巢切除组 ,卵巢切除后单纯喂给氟组及卵巢切除后喂给氟加钙组。喂养 6个月后 ,处死动物 ,测定血中的脂质过氧化物含量。结果　卵巢切除组血中LPO含量明显高于对照组 (P <0 0 5 )。卵巢切除同时喂给不同剂量的氟及氟加钙组 ,大鼠血清LPO明显低于单纯卵巢切除组 (P <0 0 1)。卵巢切除同时投给不同剂量的氟与卵巢切除同时投给不同剂量的氟加钙组比较大鼠血清LPO改变不明显 (P >0 0 5 )。结论　卵巢切除骨质疏松大鼠血中LPO明显增高 ,加入不同剂量的氟及钙后改善了去势大鼠的脂质过氧化程度。Objective To evaluate the effects of fluoride and fluoride plus calcium on serum lipid peroxide in ovariectomized rats. Methods Sixty|four female Wister rats, three months old, were divided randomly into sham|operated group(sham),ovariectomized group(OVX),ovariectomized and fed with fluoride group(OVXF),and ovariectomized and fed with fluoirde plus calcium group(OVXFCa).OVXF group was given flourid-added water after ovariectomiy once every other day, the fluorine ion contents in water being 2mg,4mg and 6mg per liter.The OVXFCa group was given fluoride in the same way as for the OVX group, with addition of calcium gluconate, simultaneously the calcium ion content in water being 900mg per liter.After six months of feeding, all animals were killed, and their serum LPO was assayed. Results There was a significant increase in serum LPO (nmol/l)in OVX group as compared with the sham group( P >0 05).The serum LPO in OVXF and OVXFCa groups was significantly lower than that in OVX group( P<0^01 ),but there was no signiticant difference between OVXF group and OVXFCa group in serum LPO ( P >0^05). Conclusion Serum LPO in ovariectomized osteoporotic rats increases significantly, and the degree of lipid peroxidation in ovariectomized rats can be improved after administration of different doses of fluoride and fluoride plus calcium.

关 键 词：卵巢切除 骨质疏松 脂质过氧化反应 氟化物 钙 

分 类 号：R681[医药卫生—骨科学]