外源性镉金属硫蛋白对小鼠脾淋巴细胞功能影响的研究  

Study of Exogenous Cadmium metallothionein on Splenic Lymphocyte Function in Mice.

作　　者：林忠宁[1] 董胜璋[1] 林育纯[1] 任铁玲[1] 余贵英[1] 董书芸[1] 

机构地区：[1]中山医科大学公共卫生学院,广州510089

出　　处：《中国公共卫生》2001年第5期410-411,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目!(基金编号No .39770 636)

摘　　要：目的　分析外源性镉金属硫蛋白 (CdMT)的免疫毒性。方法　采用分离纯化经诱导合成的CdMT作为受试物与CdCl2 比较 ,经口给予BALB/C小鼠不同剂量染毒 2周和 4周 ,检测脾T淋巴细胞增殖和T细胞亚群评价细胞免疫功能。结果　与阴性对照和脱金属硫蛋白 (Apo MT)组相比 ,CdMT组和CdCl2 组表现为T细胞增殖功能降低 ;L3T4+亚群减少、Lyt2 +亚群升高、L3T4+/Lyt2 +比值降低 ;且在CdMT组的改变明显低于相同含镉量的CdCl2 组。结论　外源性CdMT经口作用可致体内脾淋巴细胞免疫抑制作用 ,并与染毒剂量和时间有关 ;但CdMT经口免疫毒性低于CdCl2 ;Objective To analyze the immunotoxicity of exogenous cadmium metallothionein(CdMT).Methods The induced CdMT was purified and used as test substance and compared with CdCl 2.The experimental animal model was established with BALB/C mice administered by gavage daily with different dosage of CdMT or CdCl 2 for 2 and 4 weeks.The proliferation and T subsets of splenic lymphocyte of treated and control groups were examined.Results Compared with the negative control and Apo MT group,the proliferation of T lymphocyte of the CdMT and CdCl 2 groups was inhibited and the ratio of T subsets changed.The effects of CdMT on lymphocyte function was significantly weaker than those of CdCl 2 containing the same amount of Cd 2+ .Conclusion The exogenous CdMT has lymphocyte immunotoxicity effects on the animals administered via gastro intestine tract with a dose and time dependent relationship.Moreover,the effects of CdMT were obviously different from that of CdCl 2.These results provide experimental basis for explaining the biological effects of environmental cadmium exposure as well as for conducting risk assessment of cadmium.

关 键 词：镉 金属硫蛋白 免疫毒性 脾淋巴细胞功能 

分 类 号：Q355[生物学—遗传学] R995[医药卫生—毒理学]